葡萄籽提取物原花青素一般药理学研究  

Study on General Pharmacology of Grape Seed Proanthocyanidin Extract

作　　者：陈会丛[1] 翟建英[1] 张广平[1] 朱晓光[1] 杨海润[1] 张思玉[1] 蒋威[1] 李晋生[1] 

机构地区：[1]北京中研同仁堂医药研发有限公司,中药复方新药开发国家工程研究中心,北京100079

出　　处：《中国中医药信息杂志》2014年第3期36-39,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家高技术研究发展计划(2010AA023006)

摘　　要：目的观察葡萄籽提取物原花青素对实验动物心血管系统、中枢神经系统和呼吸系统的影响,为其进一步开发和使用提供一般药理学研究安全性评价实验数据。方法采用十二指肠给药方式观察原花青素对麻醉犬呼吸频率和潮气量、收缩压、舒张压、平均动脉压、心率、心电图的影响;采用灌胃给药方式观察原花青素对小鼠自主活动行为和协调运动的影响,并观察原花青素对小鼠戊巴比妥钠阈剂量和阈下剂量睡眠的影响。结果原花青素(857.00、214.29、42.86 mg/kg)对麻醉犬的潮气量和频率、血压、心率、心电图均无明显影响(P>0.05);原花青素(428.57、214.29、42.86 mg/kg)对小鼠的自主活动行为和协调运动无明显影响(P>0.05),对戊巴比妥钠阈下剂量睡眠动物数和阈剂量睡眠潜伏期和睡眠时间无明显影响(P>0.05)。结论原花青素对心血管系统、中枢神经系统和呼吸系统无明显不良影响。Objective To investigate the influences of grape seed proanthocyanidin extract （GSPE） on cardiovascular system, nervous system and respiratory system of experimental animals, and provide general pharmacological data for further research and application. Methods The influences of GSPE on blood pressure, heart rate, electrocardiogram, breathing frequency and tidal volume in anesthetic dogs after duodenal administration were observed, the impacts on spontaneous activity, coordinated motion, and the sleep situation with threshold dose and subthreshold dose of pentobarbital sodium in mice after intragastric administration were observed. Results GSPE showed no side effects on blood pressure, heart rate, electrocardiogram, breathing frequency and tidal volume in anesthetic dogs at the dosage of 857.00, 214.29, 42.86 mg/kg （P〉0.05）. At the dosage of 428.57, 214.29, 42.86 mg/kg, GSPE had no obvious influence on spontaneous activities and coordinated movements in mice （P〉0.05）. GSPE did not evidently change the number of sleeping animals, the sleep latency and the sleeping duration with subthreshold dose and threshold dose of pentobarbital sodium （P〉0.05）. Conclusion GSPE has no evident adverse effects on central nervous system, cardiovascular system and respiratory system in animals.

关 键 词：原花青素 中药药理学 心血管系统 呼吸系统 中枢神经系统 犬 小鼠 

分 类 号：R285.5[医药卫生—中药学]