抗纤灵方有效单体对5/6肾切除大鼠细胞外基质成分的影响  

Effect of Effective Monomer of Kangxianling Prescription on Extracellular Matrix Components of 5/6 Nephrectomy Rat

作　　者：唐英[1] 朱祎[1] 何立群[1] 王东[1] 谢婷婷[1] 

机构地区：[1]上海中医药大学附属曙光医院,上海200021

出　　处：《中国中医药信息杂志》2014年第3期43-46,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81173219);国家科技重大专项-重大新药创制(2009ZX09311-003);上海市中医药事业发展三年行动计划(ZYSNXD-CC-YJXYY);上海高校创新团队建设项目(2009)

摘　　要：目的观察抗纤灵方有效单体对5/6肾切除模型大鼠肾功能及细胞外基质成分的影响,为抗肾纤维化有效中药的筛选提供依据。方法将60只SD大鼠随机分为正常组、模型组、大黄酚组、丹参多酚酸A组、齐墩果酸组、氯沙坦钾组。采用手术方法建立大鼠肾纤维化模型,各给药组给予相应药物干预2个月后,观察肾脏病理变化,检测大鼠肾功能水平,并采用实时定量PCR法检测大鼠肾组织的层黏连蛋白(LN)、纤维连接蛋白(FN)、Ⅲ型胶原(C-Ⅲ)、Ⅰ型胶原(C-Ⅰ)mRNA的表达。结果模型组大鼠尿素氮(BUN)和血肌酐(SCr)显著升高,与正常组比较差异有统计学意义(P<0.01);丹参多酚酸A组大鼠SCr、BUN显著下降,与模型组比较差异有统计学意义(P<0.05,P<0.01);丹参多酚酸A组与氯沙坦组比较,BUN明显降低,差异有统计学意义(P<0.01)。各单体组能不同程度降低C-Ⅲ、C-Ⅰ、LN的表达。结论抗纤灵方有效单体可通过降低细胞外基质成分的表达而起到抗纤维化、改善肾功能作用。Objective To observe the effect of effective monomer of Kangxianling prescription on renal function and extracellular matrix composition of 5/6 nephrectomy rats model, and provide basis for the screening of effective traditional Chinese medicine of anti-renal fibrosis. Methods Sixty SD rats were randomly divided into normal group, model group, chrysophanol group, salvianolate A group, oleanolic acid group and losartan group. The kidney fibrosis model was made by operation. Two months after intervented by correspong drugs, renal pathological changes of all groups were observed, the levels of renal function indexes were detected, and real-time PCR assay was used to detect laminin （LN）, fibronectin （FN）, type Ⅲ collagen （C-Ⅲ）, type Ⅰ collagen （C-Ⅰ） mRNA expression in rat kidney tissue. Results SCr and BUN in model group increased significantly compared with the normal group （P〈0.01）. SCr and BUN in salvianolate A group decreased significantly compared with the model group （P〈0.05, P〈0.01）. BUN in salvianolate A group decreased significantly compared with the losartan group （P〈0.01）. The expression of C- ,Ⅲ C-Ⅰand LN were reduced by effective monomer of Kangxianling prescription. Conclusion Effective monomer of Kangqianling prescription can inhibit renal fibrosis through reducing the expression of extracellular matrix components, thereby improve the renal function.

关 键 词：抗纤灵 肾纤维化 中药单体 层黏连蛋白 纤维连接蛋白 大鼠 

分 类 号：R285.5[医药卫生—中药学]