即时糖化血红蛋白检验的临床应用  

Clinical application of point of care HbA1c testing

作　　者：李惠琴[1] 苏晓飞[1] 谢晓竞[1] 丁波[1] 白瑞苗[1] 夏斯桂[1] 马建华[1] 

机构地区：[1]南京医科大学附属南京第一医院内分泌科,210029

出　　处：《中华全科医学》2014年第4期617-619,共3页Chinese Journal of General Practice

摘　　要：目的评价糖化血红蛋白(HbA1c)即时检验(POCT)法和高效液相色谱(HPLC)法的一致性,探讨POCT法测定HbA1c临床应用的可能性。方法随机选取42例门诊糖尿病患者进行HbA1c的HPLC法和POCT法测定,评价2种方法的一致性和HbA1c POCT法临床应用的可行性。结果对2种测定方法进行配对t检验:不分组、HbA1c≤8.0%组、HbA1c>8.0%组,P值分别为0.226、0.899、0.080,两者之间差异无统计学意义;相关分析显示2种方法相关性良好(P<0.05);Bland-Altman一致性分析显示不分组、HbA1c≤8.0%组、HbA1c>8.0%组分别有97.62%、95.45%、95.00%的点位于界限以内。结论 HPLC法与POCT法检测HbA1c结果一致性好;POCT法能满足临床应用的需求,可以作为门诊快速检测HbA1c的较好方法。Objective To evaluate the agreement of the results of HbA1 c in Point-of-Care Test （POCT） and High Per- formance Liquid Chromatography（HPLC） ,and discuss possibility of clinical application of point of care HbAlc testing. Methods HPLC and POCT were employed to test the levels of HbAlc in 42 clinic diabetic patients. The agreement of the results of both methods and possibility of clinical application of point of care HbA1 c testing were discussed. Results Paired t-test was used to analyze the test results. For ungrouped individuals, individuals with HbA1 c 〈~ 8.0% group and with HbA1 c 〉 8.0% group, P value were 0. 226,0. 899 and O. 08, respectively, there were no statistical differences be- tween the two methods. Correlation analysis showed a significant correlation between the two methods （ P 〈 O. 05 ）. Bland- Altman analysis： 97.62% ,95.45% ,95.00% of the points were located in limit among ungrouped individuals,individuals with HbA1 c ~〈8.0% group and with HbAlc 〉 8.0% group. Conclusion The agreement of POCT and HPLC was satis- factory. The point of care HbA1 c testing can meet the needs of clinical application and used for the clinic rapid detection of HbAlc.

关 键 词：糖尿病 糖化血红蛋白 即时检验 

分 类 号：R446.11[医药卫生—诊断学] R587.1[医药卫生—临床医学]