移动克雷伯菌临床分离株氟喹诺酮类耐药机制研究  

The mechanism of resistance of klebsiella mobilis to fluoroquinolones

作　　者：许兆军[1] 许小敏[2] 刘鹏[1] 张玉楚[1] 陈童恩[1] 杨群[1] 

机构地区：[1]浙江省宁波市第二医院急诊科,315010 [2]浙江省宁波市第二医院检验科,315010

出　　处：《中华全科医学》2014年第4期661-662,共2页Chinese Journal of General Practice

基　　金：浙江省宁波市自然科学基金(2010A610037)

摘　　要：目的研究移动克雷伯菌临床分离株氟喹诺酮类耐药机制。方法采用仪器及K-B法测定抗菌药物的敏感性,聚合酶链反应(PCR)检测靶位DNA回旋酶编码基因(gyrA)与拓扑异构酶Ⅳ编码基因(parC)之氟喹诺酮耐药决定区突变分析,以及可移动遗传元件可介导的喹诺酮类耐药基因。结果 24株菌株的gyrA、parC基因氟喹诺酮耐药决定区(QRDR)10株无突变,该10株中9株环丙沙星与左氧氟沙星均为敏感。14株gyrA、parC基因氟喹诺酮耐药决定区(QRDR)存在有义突变中有13株表现为环丙沙星和/或左氧氟沙星耐药。可移动遗传元件可介导的喹诺酮类耐药基因检测均为阴性。结论移动克雷伯菌的耐氟喹诺酮类药物原因主要gyrA、parC基因突变所致。无可移动遗传元件可介导的喹诺酮类耐药基因。Objective To study The mechanism of resistance of Klebsiella mobilis to Fluoroquinolones. Methods Sensi- tivity analysis system and K-B tests was performed to detect the susceptibility. The region of gyrA, parC and mobile genetic elementsof quinolone resistance genes were amplified by PCR. Results 24 strains resistant mobile gyrA, parC gene of quinolone resistance determining region （QRDR） of 10 strains without mutation ,9 strains were sensitive to ciprofloxacin and levofloxacin. Among the 14 strains, gyrA, par（： gene of quinolone resistance determining region （QRDR） mutation in the presence of 13 strains to ciprofloxacin and/or levofloxacin resistance. Quinolone resistant genes can be mediated by mobile genetic elements test were negative. Conclusion The main mobile Klebsiella mobilis＇ resistance to fiuoroquinolo- nes was resulted from the gene encoding gyrA, parC. Quinolone resistance gene in Klebsiella mobilis had no movement of mobile genetic.

关 键 词：移动克雷伯菌 氟喹诺酮 耐药机制 PCR 

分 类 号：R517.6[医药卫生—内科学] R969[医药卫生—临床医学]