补肾健脾化瘀方对去势大鼠股骨区域性骨矿含量和骨密度的影响  

Effect of kidney-tonifying,spleen-strengthening,and blood stasis removing herbal on femoral region bone mineral content and density in an ovariectomized rat model

作　　者：黄莉[1] 刘允[2] 魏秋实[3] 邓伟民[1] 王海彬[2] 孙伟珊[1] 霍少川[2] 唐红宇[2] 

机构地区：[1]广州军区广州总医院康复科,广州510010 [2]广州中医药大学国家重点学科中医骨伤科重点实验室,广州510405 [3]广州军区广州总医院博士后科研工作站,广州510010

出　　处：《广东医学》2014年第4期489-492,共4页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:81273778);2012年广东省第一批产业技术研究与开发资金计划项目(编号:2012B050600026)

摘　　要：目的探讨补肾健脾化瘀方对去势骨质疏松大鼠区域性骨矿含量(BMC)和骨密度(BMD)的影响。方法 30只7月龄SD雌性大鼠随机分成对照组、模型组、中药低剂量组、中药高剂量组、福美加组。除对照组外,其余各组均建立去势骨质疏松大鼠模型。建模后,中药低、高剂量组分别给予0.83、2.5 mg/kg剂量的补肾健脾化瘀方,每天灌胃1次;福美加组给予5 mg/kg福美加,每周灌胃1次;对照组、模型组灌服10 mL/kg生理盐水,1次/d。给药期间记录大鼠体重变化。给药8周后,收集各组大鼠左侧股骨分别行BMC和BMD检测。结果各组大鼠给药前后体重变化差异无统计学意义(P>0.05)。模型组总BMC和BMD低于对照组(P<0.05);对照组、中药高剂量组和福美加组总BMD高于模型组和中药低剂量组(P<0.05),而对照组、中药高剂量组与福美加组之间差异无统计学意义(P<0.05)。5组间股骨内侧髁BMC和BMD差异无统计学意义(P>0.05)。股骨头、股骨干BMC和BMD以及大转子BMD,模型组、中药低剂量组低于其余3组(P<0.05),而对照组、中药高剂量组与福美加组比较差异无统计学意义(P>0.05)。对照组和福美加组大转子BMC高于其余3组(P<0.05)。中药高剂量组股骨外侧髁BMC高于其余4组(P<0.05),对照组和中药高剂量组股骨外侧髁BMD高于其余3组(P<0.05)。结论去势大鼠股骨不同区域均存在BMC和BMD下降,补肾健脾化瘀方可提高全段股骨BMC和BMD,尤其对提高股骨外侧髁BMC和BMD更有优势。Objective To investigate the effects of kidney-tonifying, spleen-strengthening, and blood stasis removing herbal on femoral region bone mineral content and density in an ovariectomized rat model.Methods Thirty female non-pregnant SD rats （7 months） were randomly divided into 5 groups： control group, ovariectomized group, low-dose herb group, high-dose herb group and Fumeijia group. Ovariectomized rat models were set up in all the other groups except for control group. Low-dose （0.83 mg/kg） or high-dose （2.5 mg/kg） Bushenzhuanggu Granule ig qd. was given to low-dose herb group or high-dose herb group, respectively; while fumeijia （5mg/kg） was given to Fumeijia group weekly. Rat weights were recorded during treatment. After the 8-week treatment, the bone mineral density （BMD） and bone mineral content （BMC） were measured by small animals specially dual energy X-ray absorptiometry.Results There was no significant difference on pre-/post-treatment weight changes among five groups. Compared with control group, the BMC and BMD in ovariectomized group were significantly reduced （P〈0.05）. Compared with ovariectomized group and low-dose herb group, the BMD of high-dose herb group and Fumeijia group were significantly increased （P〈0.05）; while no significant difference was observed among control, high-dose herb and Fumeijia groups. There was no significant difference on BMC or BMD of femoral medial condyle among the five groups. Compared with ovariectomized group and low-dose herb group, significant increase in the BMC and BMD of femoral head and shaft, the BMD of greater trochanter in control, high-dose herb and Fumeijia groups （P〈0.05）. Compared with ovariectomized, low-dose herb and high-dose herb groups, significant increase in the BMC of greater trochanter were obserrved in control and Fumeijia group （P〈0.05）. Compared with ovariectomized, low-dose herb and Fumeijia groups, significant increase in the BMC and BMD of femoral lateral condyle was observed in high-dose herb

关 键 词：中药 去势大鼠 骨质疏松 区域性骨密度 

分 类 号：R285[医药卫生—中药学]