子宫自然杀伤细胞在子痫前期母胎界面的分布变化  

Uterine natural killer cells distribution in the maternal-fetal interface in preeclampsia

作　　者：宗滕[1] 钟婷[1] 郭美君[1] 赖丽丹 张保平[2] 任敏[2] 俞晓春[2] 况海斌[1] 

机构地区：[1]南昌大学基础医学院,南昌330006 [2]南昌大学医学实验教学部,南昌330006

出　　处：《广东医学》2014年第4期513-516,共4页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:31260248;81270668;30960118);江西省研究生创新资金资助项目(编号:YC2012-S022)

摘　　要：目的探讨子痫前期患者妊娠母胎界面的子宫自然杀伤细胞(uNK细胞)的分布变化以及其与临床症状的关系。方法用uNK细胞特异性的DBA-lectin抗体,对40例子痫前期患者和20例正常足月妊娠妇女妊娠母胎界面的蜕膜和绒毛组织进行免疫组织化学标记。结果 (1)在正常妊娠的母胎界面,与妊娠早期的蜕膜组织中uNK细胞数相比,妊娠晚期蜕膜组织中的uNK细胞数量明显减少(P<0.01),且在妊娠晚期的绒毛组织中,不表达uNK细胞。(2)与正常妊娠晚期的蜕膜组织相比,子痫前期患者妊娠晚期的蜕膜组织中uNK细胞数量明显增多(P<0.01)。而在绒毛组织中,与正常妊娠晚期绒毛组织一样,子痫前期患者的绒毛组织也不表达uNK细胞。(3)子痫前期患者蜕膜组织中uNK细胞lectin染色阳性的光密度值与平均动脉压呈正相关(r=0.72,P=0.021),而与24 h尿蛋白量没有明显的相关性(r=0.340,P=0.198)。结论蜕膜组织中的uNK细胞表达失衡在子痫前期发病中起重要作用,且与患者的平均动脉压密切相关。Objective To investigate the distribution changes of uterine natural killer cells （uNK cells） in the maternal-fetal interface in preeclampsia and its correlation with clinical symptoms.Methods The unique uNK cell marker, Dolichos biflorus agglutinin （DBA） lectin antibody, was applied for immunohistochemical staining, thus to localize uNK cells in the decidual and villous tissues of pregnant maternal-fetal interface. Results In normal pregnancy, uNK cell count of the deciduas in the late pregnancy was significantly reduced when compared with decidual samples in early pregnancy （P〈0.01）; and no uNK cell was observed in chorionic villi in late pregnancy. Compared with the deciduas of normal late pregnancy, decidual uNK cells count in the third trimester of preeclampsia patients was significantly increased （P〈0.01）. Furthermore, no uNK cell was observed in villi in preeclampsia or normal pregnancy in the third trimester. Lectin staining optical density of decidual uNK cell in the third trimester in preeclampsia was significantly positively correlated with mean arterial pressure （r=0.72, P=0.021）, but not 24-hour urine protein （r=0.340, P=0.198）.Conclusion The expression imbalance of preeclamptic decidual uNK cells may play an important role in the pathogenesis of preeclampsia, and correlate to the patient′s mean arterial pressure.

关 键 词：先兆子痫 子宫自然杀伤细胞 Lectin染色 母胎界面 妊娠 

分 类 号：R714.21[医药卫生—妇产科学]