模拟高原训练对大鼠小肠粘膜屏障的影响及其小麦肽的干预作用  

The Effects of Simulated Altitude Training on Intestinal Mucosa Barrier in Rats and the Intervention of Wheat Peptide

作　　者：金其贯[1] 佘奇[1] 金爱娜[1] 潘兴昌[2] 刘霞[1] 蔡木易[2] 

机构地区：[1]扬州大学体育学院,江苏扬州225009 [2]中国食品发酵工业研究院,北京100027

出　　处：《西安体育学院学报》2014年第2期225-230,共6页Journal of Xi'an Physical Education University

基　　金：中国食品发酵工业研究院横向基金课题

摘　　要：目的探讨高原训练对大鼠小肠粘膜形态结构和功能的影响及可能机制,并探讨小麦肽的干预作用。方法清洁级雄性SD大鼠随机分成正常对照组(C组,n=10)、运动训练组(E组,n=10)、低氧对照组(HC组,n=10)、低氧+运动训练组(HE组,n=10)和低氧+运动训练+小麦肽组(HEW组,n=10)5组。低氧条件为模拟海拔高度3 000 m,氧浓度为14.2%。运动训练采用90 min的无负重游泳运动,每周6天。HEW组在每次训练后按照500 mg/kg·bw剂量灌服小麦肽溶液。9周后,观察小肠粘膜组织结构,并检测大鼠血清MAO含量以及肠组织中SOD、GSH-Px、MDA的含量。结果 (1)长期的运动训练可使大鼠小肠绒毛长度和数量增加,血清MAO活性降低,但无显著性差异(P>0.05),而低氧暴露可使大鼠小肠绒毛长度和数量均显著减少,血清MAO活性显著升高(P<0.01),低氧和运动训练对进一步降低大鼠小肠绒毛长度、数量和升高血清MAO活性具有显著的交互作用(P<0.05)。与HE相比,HEW组大鼠小肠绒毛长度和数量均显著增加(P<0.05),血清MAO活性极显著性降低(P<0.01)。(2)长期的运动训练可使大鼠小肠SOD、GSH-Px活性升高,MDA含量降低,但无显著性差异(P>0.05),低氧暴露可使大鼠小肠SOD、GSH-Px活性显著降低,MDA含量显著升高(P<0.01),低氧暴露和运动训练对进一步降低大鼠小肠SOD、GSH-Px活性,升高MDA含量具有显著的交互作用(P<0.01)。与HE组相比,HEW组小肠SOD和GSH-Px活性显著升高(P<0.05,P<0.01),MDA含量显著降低(P<0.01)。结论 (1)长期的高原训练能引起肠道抗氧化能力下降,氧自由基生成增多,从而导致小肠粘膜的结构和功能发生损伤。但在低氧和运动训练两个因素中,低氧对小肠粘膜屏障的影响占有主导地位。(2)补充小麦肽可提高高原训练大鼠小肠的抗氧化能力,减少氧自由基的生成,有效地防止长期高原训练引起的小肠粘膜屏障的损伤。Objective In order to explore the effect of simulated altitude training on intestinal mucosa morphosis and function in rats and the possible mechanism, and the intervention of wheat peptide supplement on it. Methods 50 SD rats were ran- domly divided into normal control group （group C, n = 10）, hypoxia control group （group HC, n = 10）, exercise training group （group E,n = 10）,hypoxia ＋ exercise training group（group HE,n = 10） and hypoxia ＋ Exercise ＋ wheat peptide group （ group HEW, n = 10） 5 groups. Hypoxic condition was simulated altitude 3 000 m, oxygen concentration was 14.2%. Exer- cise training using 90 min unload swimming exercise,6 days a week. Wheat peptide solution was gastric lavaged in the HEW group after each training in accordance with the dose of 500 mg/kg BW. After 9 weeks,the intestinal mucosa morphosis, ser- um MAO activity and intestinal SOD, GSH -Px activities, MDA content were measured. Results （1）The long- term exer- cise training could increase the length and the number of small intestinal villus, decreased serum MAO activity, but no signifi- cant difference （ P 〉 0.05 ） , while hypoxia exposure decreased the length and the number of small intestinal villus significant- ly,increased serum MAO activity significantly （P 〈 0.01 ） , hypoxia and exercise training had a significant interaction to fur-ther reduce the length, number of intestinal villus and increase serum MAO activity（P 〈 0.05 ）. Compared with group HE, the length and number of small intestine villus in group HEW were increased significantly （ P 〈 0.05 ）, serum MAO activity was significantly decreased （P 〈 0.01 ）. （2）The long- term exercise training could increase the intestinal SOD, GSH -Px activity and decrease MDA content, but there were no significant difference （P 〉 0.05 ）, hypoxia exposure could decrease the intestinal SOD,GSH- Px activity significantly, and increase MDA content significantly （P 〈 0.01 ）, hypoxia and exercise training has a significan

关 键 词：高原训练 小麦肽 小肠粘膜屏障 氧自由基 

分 类 号：G804.7[文化科学—运动人体科学]