Transcriptomic analysis of Synechocystis sp. PCC6803 under low-temperature stress  

作　　者：刘志香 崔红利 刘正一 王寅初 崔玉琳 刘兆普 秦松 

机构地区：[1]College of Resources and Environmental Sciences,Key Laboratory of Marine Biology,Nanjing Agricultural University [2]Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences

出　　处：《Chinese Journal of Oceanology and Limnology》2014年第2期403-418,共16页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.40876082);the International Innovation Partnership Program:Typical Environmental Process and Effects on Resources in Coastal Zone Area;the Public Science and Technology Research Funds Projects of the Ocean(Nos.200905021,201205027);the Outstanding Young Scholars Fellowship of Shandong Province(Molecular Phycology,No.JQ200914);the Natural Science Foundation of Shandong Province(No.ZR2012DQ015);the Guangdong Province Comprehensive Strategic Cooperation Project of the Chinese Academy of Sciences(No.2011A090100040)

摘　　要：In this study, cDNA microarrays were developed from 3569 mRNA reads to analyze the expression profiles of the transcriptomes of Synechocystis sp. PCC6803 under low temperature （LT） stress. Among the genes on the cDNA microarrays, 899 LT-affected genes exhibited a 1.5-fold （or greater） difference in expression compared with the genes from normal unstressed Synechocystis sp. PCC6803. Of the differentially expressed genes, 353 were up-regulated and 246 were down-regulated. The results showed that genes involved in photosynthesis were activated at LT （10℃）, including genes for photosystem I, photosystem II, photosynthetic electron transport, and cytochrome b6/f complex. Moreover, desg, one of four genes that encode the fatty acid desaturases, was also induced by LT. However, the LT conditions to some degree enhanced the transcription of some genes. In addition, LT （10℃） may reduce cellular motility by regulating the transcription of spkA （sll1575）, a serine/threonine protein kinase. The results reported in this study may contribute to a better understanding of the responses of the Synechocystis cell to LT, including pathways involved in photosynthesis and repair.In this study,cDNA microarrays were developed from 3569 mRNA reads to analyze the expression profi les of the transcriptomes of Synechocystis sp.PCC6803 under low temperature(LT)stress.Among the genes on the cDNA microarrays,899 LT-affected genes exhibited a 1.5-fold(or greater)difference in expression compared with the genes from normal unstressed Synechocystis sp.PCC6803.Of the differentially expressed genes,353 were up-regulated and 246 were down-regulated.The results showed that genes involved in photosynthesis were activated at LT(10°C),including genes for photosystem I,photosystem II,photosynthetic electron transport,and cytochrome b6/f complex.Moreover,desB,one of four genes that encode the fatty acid desaturases,was also induced by LT.However,the LT conditions to some degree enhanced the transcription of some genes.In addition,LT(10°C)may reduce cellular motility by regulating the transcription of spkA(sll1575),a serine/threonine protein kinase.The results reported in this study may contribute to a better understanding of the responses of the Synechocystis cell to LT,including pathways involved in photosynthesis and repair.

关 键 词：Synechocystis sp. PCC6803 CYANOBACTERIA cDNA microarray TRANSCRIPTOMICS low temperature stress 

分 类 号：Q943[生物学—植物学]