非梗阻性无精子症患者睾丸生精细胞体外培养后受精能力的研究  

Fertilization ability of spermatogenic cells from nonobstructive azoospermia patients after culture in vitro

作　　者：檀大羡 莫毅 陈自洪 张海英 刘琼东 牛向丽 孙燕萍 

机构地区：[1]广西壮族自治区人口和计划生育委员会生殖中心,南宁530021

出　　处：《中国性科学》2014年第2期3-4,共2页Chinese Journal of Human Sexuality

基　　金：广西壮族自治区计划生育委员会科研课题资助(合同号:桂人口计生研0503)

摘　　要：目的:探讨非梗阻性无精子症患者睾丸生精细胞体外培养后的受精能力。方法:选取8例非梗阻性无精子症患者的睾丸组织制备成生精细胞,经体外培养后得到长形精子细胞显微注射到卵细胞内,观察其受精情况。结果:8例患者睾丸生精细胞经体外培养后均有不同程度的分化、成熟。将培养后接近成熟的精子细胞通过显微注射到卵细胞内,卵细胞受精率为4.52%,荧光原位杂交试验结果显示卵细胞有受精。结论:非梗阻性无精子症患者睾丸生精细胞经体外培养后有潜在的受精能力。Objectives： To explore the fertilization ability of spermatogenic cells cultured in vitro taken from nonobstructive azoospermia patients. Method： Spermatogenic cells were taken from testicular tissues of eight nonobstructive azoospermia patients, and were cultured in vitro. The elongated spermatids from spermatogenic cells cultured in vitro were microinjected into oocytes. Fertilization process and outcome were observed. Results： There were differentiation and maturation of spermatogenic cells of all eight patients after being cultured in vitro. The fertilization rate was 4.52%. The fluorescence in situ hybridization （FISH） test results showed that the oocytes were fertilized. Conclusion： Spermatogenic cells cultured in vitro of patients with nonobstructive azoospermia have the potential fertilization ability.

关 键 词：无精子症 生精细胞 体外培养 显微受精 荧光原位杂交 

分 类 号：R697.22[医药卫生—泌尿科学]