正交试验优选芪丹颗粒提取工艺  

Optimization of Extraction Process of Qidan Granules by Orthogonal Test

作　　者：袁天荣[1,2] 赵雪梅[1] 赵蕾[2] 董琼[2] 

机构地区：[1]山东大学附属省立医院,济南250021 [2]山东中医药大学,济南250355

出　　处：《中国实验方剂学杂志》2014年第7期4-8,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省中医药科技发展计划项目(2009-148)

摘　　要：目的:优选芪丹颗粒的提取工艺。方法:以丹酚酸B、丹参酮ⅡA质量分数和干膏率为评价指标,通过正交试验考察乙醇体积分数、提取次数、乙醇用量对醇提工艺的影响;以黄芪甲苷提取量和出膏率为指标,通过正交试验考察煎煮时间、煎煮次数、加水量对水提取工艺的影响。采用HPLC测定黄芪甲苷、丹酚酸B及丹参酮ⅡA含量。结果:最佳醇提工艺为加6倍量60%乙醇回流提取2次,提取时间分别为2,1 h;丹酚酸B和丹参酮ⅡA质量分数分别为1.215%,0.599%,干膏率41.04%。最佳水提工艺为加10倍量水煎煮2次,每次1.5 h;黄芪甲苷提取量3.408 mg·g-1,出膏率43.37%。结论:优选的提取工艺稳定可行,为芪丹颗粒的合理开发提供参考。Objective： To optimize extraction process of Qidan granules. Method： Taking tanshinone ⅡA content,salvianolic acid B content and dry extract rate as indexes,orthogonal test was adopted to optimize alcohol extraction process with extraction times,the concentration and consumption of ethanol as factors;With dry extract rate and extracting amount of astragaloside as indexes,orthogonal test was adopted to investigate effects of the amount of water,boiling time and decocting times on water extraction process.Contents of astragaloside,salvianolic acid B and tanshinone ⅡA were determined by HPLC. Result： Optimum alcohol extraction conditions were as follows：extracted twice with six times the amount of 60% ethanol,extraction time of 2,1 h;Dry extract rate was 41.04%,mass fraction of salvianolic acid B and tanshinone ⅡA were 1.215% and 0.599%,respectively.Optimum water extraction conditions were as follows：decocted twice with ten times the volume of water,1.5 h per time;Extracting amount of astragaloside was 3.408 mg·g^-1,dry extract rate was 43.37%. Conclusion： Optimized extraction process was stable and feasible,which could provide a reference for rational development of Qidan granules.

关 键 词：芪丹颗粒 正交试验 醇提工艺 水提工艺 丹酚酸B 丹参酮ⅡA 干膏率 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]