铁皮石斛超微粉碎破壁工艺的优化  

Optimization of Ultrafine Grinding Technology for Cell Wall Disruption of Dendrobii Officinalis Caulis

作　　者：李娟[1] 麻晓雪[1] 李顺祥[1] 蔡光先[1] 张家曾 

机构地区：[1]湖南中医药大学中药现代化教育厅重点实验室,长沙410208

出　　处：《中国实验方剂学杂志》2014年第7期30-33,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖南省科技计划重大专项项目(2010FJ1010-3);国家中医药管理局"药用植物学"重点学科课题(国中医药发[2009]30号);湖南"中药学"重点学科(湘教通[2011]76号);湖南省高校科技创新团队项目(湘教通[2010]212号)

摘　　要：目的：优选铁皮石斛的超微粉碎工艺。方法：以微粉粒度、细胞破壁率和总多糖含量为综合评价指标，采用L16（43×26）正交试验考察入磨量、粉碎时间、入磨粒度、粉碎温度和投料水分对铁皮石斛超微粉碎破壁工艺的影响。结果：最佳超微粉碎工艺为入磨粒径80目，投料水分4%-6%，入磨量250 g，粉碎温度0-10 ℃，粉碎时间25 min；平均得粉率96.1%（RSD 0.24%），粒径〈75 μm的平均得率96.8%（RSD 0.28%），平均细胞破壁率98.3%（RSD 0.78%），总多糖平均质量分数42.2%（RSD 2.85%）。结论：该工艺合理可行、稳定性好，为铁皮石斛的临床合理应用提供实验数据。Objective： To optimize ultrafine grinding technology for cell wall disruption of Dendrobii Officinalis Caulis. Method： With particle size,cell wall-broken rate and the content of total polysaccharides as comprehensive evaluation index,L16（43×26） orthogonal test was adopted to optimize ultrafine grinding process by taking grinding time,amount and particle size into mill,grinding temperature and moisture content of materials as factors. Result： Optimum grinding process was as follows：particle size into mill 80 mesh,moisture content of materials 4%-6%,materials amount into mill 250 g,grinding temperature 0-10 ℃,grinding time 25 min;Average powder yield was 96.1%（RSD 0.24%）,average yield of particle size〈75 μm was 96.8%（RSD 0.28%）,average cell wall-broken rate was 98.3%（RSD 0.78%）,average mass fraction of total polysaccharides was 42.2%（RSD 2.85%）. Conclusion： This optimized process was reasonable and stable,which provided experimental data for clinical effective application of Dendrobii Officinalis Caulis.

关 键 词：铁皮石斛 超微粉碎工艺 细胞破壁 粒径分布 正交试验 综合加权法 

分 类 号：R283.3[医药卫生—中药学]