不同生长期灯心草皮部和髓部中去氢厄弗酚含量比较  

Analysis on Dehydroeffusol Content of Cortex and Pith of Juncus effusus in Different Growing Stage

作　　者：郭珍玉 李贵云[1] 孙雪[1] 段天璇[1] 黄建梅[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国实验方剂学杂志》2014年第7期104-106,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173541)

摘　　要：目的:测定和比较两个产地不同生长时间灯心草皮部和髓部中去氢厄弗酚(DHE)的含量。方法:采用高效液相色谱(HPLC),Agilent Eclipse Plus-C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-水(57∶43),流速1.0 mL·min-1,检测波长270 nm,柱温30℃。结果:灯心草髓部中DHE的含量均明显高于同期皮部;两产地灯心草髓部中去氢厄弗酚的含量,在生长后期差异增大;两产地各时期灯心草皮部中去氢厄弗酚的含量较低,但在灯心草全草中,皮部所含DHE量与髓部相当。结论:H省和J省灯心草中DHE含量差异较大。Objective： To determine and compare the dehydroeffusol（DHE） content in rush pith and cortex of Juncus effusus collected in 2 places and different time. Method： The content of dehydroeffusol was determined by HPLC.An Agilent Eclipse Plus-C18 column （4.6 mm×250 mm,5 μm） was employed, the mobile phase of MeOH-H2O（57：43） was used at the flow rate 1.0 mL·min^-1 and the signal was detected at 270 nm. Result： The content of DHE in pith was significantly higher than that in cortex in the same period;the difference of DHE contents given by the 2 producing areas increased with time;despite the cortex of J. effusus was contained little DHE per gram, it roughly contributed as much as DHE which contributed by the pith in the whole plant. Conclusion： The quality of J. effusus in 2 places have large differences.

关 键 词：灯心草 高效液相色谱 生长时期 去氢厄弗酚 含量测定 

分 类 号：R284.1[医药卫生—中药学]