在体单向肠灌流模型研究红景天苷的大鼠肠吸收特性  

Intestinal Absorption of Salidroside by Rats Single Pass Perfusion in situ

作　　者：吴浩[1,2] 狄留庆[1,2] 单进军[3] 康安[1] 赵晓莉[1,2] 祖强[1,2] 

机构地区：[1]南京中医药大学药学院,南京210023 [2]江苏省中药高效给药系统工程技术研究中心,南京市中药微丸产业化工程技术研究中心,南京210023 [3]南京中医药大学第一临床医学院,南京210023

出　　处：《中国实验方剂学杂志》2014年第7期130-134,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：教育部科学技术研究重点项目(208051);江苏省高校"青蓝工程"科技创新团队支持计划[苏教师(2008)30号];江苏省优势学科支持计划(YSXK-2010)

摘　　要：目的：研究红景天苷在大鼠小肠的吸收特征。方法：运用大鼠在体单向肠灌流模型，以酚红为标示物，分别研究了不同浓度、肠段红景天苷的吸收情况。结果：红景天苷在pH 5.5-8.0的Krebs-Ringer（K-R）试液中稳定性较好。不同浓度的红景天苷在大鼠各肠段的Peff无显著性差异，表明红景天苷在大鼠小肠内吸收无明显变化，且无自身浓度抑制作用，主要以被动转运方式吸收。十二指肠、空肠、回肠、结肠的肠灌流的有效渗透系数（Peff）、吸收速率参数（Ka）值表明，红景天苷在十二指肠、空肠、回肠的吸收优于结肠。结论：红景天苷在大鼠小肠吸收方式以被动转运为主，且在肠道中易吸收。Objective： To study the intestines absorption mechanism of salidroside using signal-pass perfusion model in rats. Method： After one-way intestinal perfusion model of rat in situ was set up, by taking phenolsulfonphthalein as a marker, the absorption of salidroside in small intestine under conditions of different concentrations, segments, and pH values was studied respectively. Result： Salidroside was much stable in Krebs-Ringer（K-R） solution, pH value was 5.5-8.0. No significant difference of Peff value was found in different concentrations of salidroside at different intestine segments, which showed that there was no obvious change in absorption of salidroside in rat small intestine and no inhibition due to different concentrations and the absorption of salidroside mainly was complied with the passive diffusion. The Peff and Ka values of intestinal perfusion showed that the absorption of salidroside in duodenum, jejunum, and colon were better than that of ileum. Conclusion： Salidroside absorption in rat intestine is mainly by passive diffusion, and is easily absorbed in the intestine.

关 键 词：红景天苷 在体单向肠灌流 吸收部位 吸收机制 

分 类 号：R285.5[医药卫生—中药学]