九节木不同极性提取物对小鼠学习记忆能力的影响  

Effect of Different Polar Extracts from Psychotria rubra on Learning and Memory Abilities of Mice

作　　者：卢海啸[1,2] 李家洲[2] 勾玲[2] 邓恒泉 李典鹏[1] 

机构地区：[1]广西壮族自治区中国科学院广西植物研究所,广西植物功能物质研究与利用重点实验室,广西桂林541006 [2]玉林师范学院,广西玉林537000

出　　处：《中国实验方剂学杂志》2014年第7期140-143,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西植物功能物质研究与利用重点实验室开放基金(FPRU2013-5)

摘　　要：目的:探讨九节木不同极性提取物对小鼠学习记忆能力的影响。方法:采用95%乙醇进行提取,然后依次用石油醚、乙酸乙酯、正丁醇进行萃取,得到各部分提取物,各种提取物灌胃给药的剂量为200 mg·kg-1,脑复康组的给药剂量为120 mg·kg-1。运用跳台法检测九节木不同萃取物对盐酸氯丙嗪、亚硝酸钠、乙醇、东莨菪碱所致记忆障碍小鼠学习记忆能力的影响,并筛选出活性部位。结果:九节木的提取物能延长小鼠跳台实验的潜伏期,减少错误次数,活性部位为乙酸乙酯部位(P<0.05)。结论:九节木的乙酸乙酯提取物和水提取物具有改善学习记忆障碍小鼠学习记忆能力的作用,其中乙酸乙酯部位为活性部位,且其作用呈现一定的量效关系。Objective： To assess the effect of different polarity extracts from Psychotria rubra on learning and memory abilities of mice. Method： P. rubra was extracted by 95% ethanol, and then was extracted by petroleum ether, ethyl acetate and butanol respectively. The dose of different polar extracts and piracetam was 200, 120 mg·kg^-1,respectively. Effect of different polarity extracts from P. rubra on learning and memory abilities was tested in the mice model caused by chlorpromazine hydrochloride, sodium nitrite, ethanol and scopolamine,respectively, and screened the active part of P. rubra. Result： Extracts from P. rubra could prolonged latency and decreased error frequency of mice. Conclusion： The ethyl acetate extracts and the water extracts of P. rubra can improve the learning and memory abilities and the ethyl acetate extract may be an active part.

关 键 词：九节木 学习记忆 活性部位 

分 类 号：R285.5[医药卫生—中药学]