健脾活血方对胃癌前病变大鼠胃黏膜COX-2及MMP-2表达的影响  

Effect of Jianpi Huoxue Decoction on COX-2 and MMP-2 Expression in Gastric Mucosa of Rats with Precancerous Lesions of Gastric Cancer

作　　者：封慧[1] 叶柏[2] 朱萱萱[2] 周杰[1] 严晶[1] 

机构地区：[1]南京中医药大学,南京210029 [2]南京中医药大学第一附属医院,南京210029

出　　处：《中国实验方剂学杂志》2014年第7期161-165,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省高校优势学科建设项目;江苏省中医药管理局课题(JD11039)

摘　　要：目的:研究健脾活血方对胃癌前病变(PLGC)大鼠胃黏膜组织中环氧合酶-2(COX-2)及基质金属蛋白酶-2(MMP-2)表达的影响。方法:60只大鼠随机分为2组:正常组10只和造模组50只。正常组正常喂养,余下大鼠每天给予150 mg·L-1的甲基硝基亚硝基胍(MNNG)溶液自由饮用,0.03%雷尼替丁加入170 mg·L-1的MNNG溶液中按10 mL·kg-1每天灌胃1次,40%的乙醇按10 mL·kg-1每周灌胃2次,连续12周,其中第5周开始结合饥饱失常,建立胃癌前病变大鼠模型。将造模成功的40只大鼠随机分为模型组(0.9%氯化钠溶液)、胃复春组(0.86 g·kg-1)、健脾活血方高、中、低剂量组(32,16,8 g·kg-1),每组8只,每组每天给予不同药物灌胃一次,连续10周。实验第22周末处死大鼠,给予相应处理后,快速免疫组化检测COX-2及MMP-2表达情况。结果:与正常组相比,模型组COX-2,MMP-2表达均显著升高(P<0.01);与模型组相比,健脾活血方高、中、低剂量组、胃复春组COX-2,MMP-2表达均显著降低(P<0.01或P<0.05);与胃复春组相比,健脾活血方高剂量组COX-2,MMP-2表达均显著降低(P<0.05),中、低剂量组COX-2,MMP-2表达与胃复春组相比无明显差异。结论:健脾活血方治疗胃癌前病变与降低胃黏膜中COX-2及MMP-2的表达相关。Objective： To investigate the effects of Jianpi Huoxue decoction on cyclooxygenase-2（COX-2） and matrix metalloproteinase-2 （MMP-2） expression in rats with precancerous lesions of gastric cancer（PLGC）. Method： Sixty rats were randomly divided into the normal group（10） and the modeling group（50）.Except normal group,PLGC model was established in other rats for 12 weeks with following. Method： 150 mg·L^-1 N-methyl-N-nitro N-nitrosoguanidine（MNNG） solusion was drunk freely,170 mg·L^-1 MNNG which contained 0.03% ranitidine at dose of 10 mL·kg^-1 was administered once a day, 40% alcohol （10 mL·kg^-1）was administered twice a week, and irregular diet was given from the 5th week.Forty rats after modeling were randomly divided into the model control group（0.9%NS）,the Weifuchun group（0.86 g·kg^-1）,the high-dose,middle-dose and low-dose of Jianpi Huoxue decoction groups（32,16,8 g·kg^-1）,8 rats in each group.Each group was given corresponding medicines once a day for 10 weeks.All of the rats were sacrificed by the 22th weekend.The expression of COX-2 and MMP-2 was detected by rapid immunohistochemistry. Result： Compared with the normal group,the COX-2 and MMP-2 expressions were significantly increased in the model group（P〈0.01）,respectively. Compared with the model group,the COX-2 and MMP-2 expressions were significantly decreased in the high-dose,middle-dose,low-dose Jianpi Huoxue decoction groups and Weifuchun group（P〈0.01 or P〈0.05）,respectively.Compared with the Weifuchun group,the COX-2 and MMP-2 expressions were significantly decreased in the high-dose of Jianpi Huoxue decoction group（P〈0.05）,respectively.But there was no significant difference in the the COX-2 and MMP-2 expression between the Weifuchun group and the middle-dose and low-dose Jianpi Huoxue decoction groups. Conclusion： The therapeutic effect of Jianpi Huoxue decoction is associated with the reduction of COX-2 and MMP-2 expression in gastric mucosa.

关 键 词：健脾活血方 胃癌前病变 环氧合酶-2 基质金属蛋白酶-2 

分 类 号：R285.5[医药卫生—中药学]