玉郎伞提取物对大鼠急性肺损伤的影响  

Effects of Yulangsan Extracts on Acute Lung Injury in Rats

作　　者：黄媛恒[1,2] 李映新[1] 谭宏棣[3] 覃斐章[1] 黄仁彬[1] 

机构地区：[1]广西医科大学药理学教研室,南宁530021 [2]广西医科大学实验生理科学实验中心,南宁530021 [3]右江民族医学院附属医院,广西百色533000

出　　处：《中国实验方剂学杂志》2014年第7期174-177,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西科学研究与技术开发计划项目(桂科攻0630002-2A);广西自然科学基金重点项目(2011GXNSFD018030);南宁市科学技术局科技攻关与新产品试制(201102084C)

摘　　要：目的:探讨玉郎伞(YLS)提取物对大鼠急性肺损伤(ALI)的影响。方法:将Wistar大鼠随机分为7组,分别为正常组,模型组,阳性对照组(地塞米松,3 mg·kg-1),玉郎伞水提物(TYLS)高、低剂量组(按生药量计60,30 g·kg-1),YLS总黄酮(FYLS)高、低剂量组(0.1,0.05 g·kg-1),每组动物连续灌胃给药7 d,于末次给药后30 min,采用腹腔注射20%酵母混悬液5 mL·kg-12次诱发大鼠急性肺损伤模型。最后测定肺组织的湿干比重(W/D)和肺组织髓过氧化物酶(MPO)的活性,免疫组化法测定大鼠肺组织中核因子-κB p65(NF-κB p65)的表达、ELISA法测定肺组织白介素-1β(IL-1β)的含量,并HE染色观察肺组织病理形态改变。结果:与正常组比较,模型组肺W/D和MPO活性增大、肺组织IL-1β含量增加、NF-κB p65表达升高(均P<0.01),肺组织有炎性病理改变。与模型组比较,TYLS高、低剂量组(4.31±0.15),(4.72±0.35)及FYLS高剂量组(4.74±0.23)肺W/D低于模型组(5.01±0.33)(P<0.05,P<0.01);TYLS高剂量组及FYLS高剂量组的肺MPO活性为(1.000±0.304),(0.956±0.139)U·g湿片-1,肺IL-1β含量为(265.58±47.05),(222.31±53.99)ng·L-1,肺组织细胞NF-κB p65阳性率为(51.00±8.62)%,(48.88±11.80)%,均比模型组明显降低(P<0.05,P<0.01);另外,从组织病理学角度上看,TYLS及FYLS组肺损伤程度也有所减轻,以高剂量组较为明显。结论:预先给予TYLS或FYLS可明显减轻ALI大鼠肺组织炎症反应及肺水肿程度,其机制可能是通过干扰NF-κB途径从而抑制IL-1β生成。Objective： To investigate the effect of Yulangsan （YLS） extracts on acute lung injury in rats. Method： The Wistar rats were randomly divided into control group, model group, dexamethasone group（3 mg·kg^-1）, high and low dose groups of total YLS water extract （TYLS） （60,30 g·kg^-1）and flavonoids extracted from the roots of YLS（FYLS）（0.1,0.05 g·kg^-1）. The animals were intragastrically treated with drugs for 7 days.The ALI model was set up by intraabdominal injection of 20% yeast suspension 5 mL·kg^-1, two time 30 minutes after the last medication.The lung wet/dry weight ratio（W/D） and the activity of myeloperoxidase（MPO） in the lung tissues were determined to assess lung injury. The expression of nuclear factor κB（NF-κB） p65 was analyzed by immunohistochemistry. The content of interleurin-1β（IL-1β）in the lung tissue was detected by ELISA. Meanwhile, the pathological change of lung was also observed by HE dying. Result： As compared with the control group, the lung W/D, MPO activity,IL-1β content and expression of NF-κB p65 were higher in the model group（P〈0.01）. HE dying showed inflammatory changes in lung in the model group. In TYLS high-and low-dose groups and FYLS high dose group,the lung W/D was （4.31±0.15）,（4.72±0.35）,（4.74±0.23） respectively, was significantly lower compared with model group（P〈0.05,P〈0.01）. The MPO activity was （1.000± 0.304）,（0.956±0.139 ）U·g^-1,IL-1β content was（265.58±47.05）,（222.31± 53.99）ng·L^-1, NF-κB p65 expression level was（51.00±8.62）%, （48.88±11.80）%, in the high dose group of TYLS and FYLS respectively. The MPO activity and IL-1β content and NF-κB p65 expression level in lung were significantly reduced compared with model group（P〈0.05,P〈0.01）.On the other hand,histopathological examination confirmed that the degree of lung injury in TYLS and FYLS groups was lessened. And high-dose groups showed the best effect. Conclusion： Preliminary administration of

关 键 词：玉郎伞 急性肺损伤 核因子-ΚB 白介素-1Β 

分 类 号：R285.5[医药卫生—中药学]