决明子乙醇提取物对高脂血症模型大鼠糖脂代谢及相关炎性细胞因子的影响  

Effects of Ethanol Extraction from Cassiae Semen on Serum IL-6 and TNF-α in Hyperlipidemia Rats

作　　者：王永辉[1] 高丽[1] 周文静[1] 马艳苗[1] 

机构地区：[1]山西中医学院,太原030024

出　　处：《中国实验方剂学杂志》2014年第7期178-181,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山西省高校研究开发项目(20111114)

摘　　要：目的:观察决明子乙醇提取物对实验性高脂血症模型大鼠血脂、血糖及炎性细胞因子白介素6(IL-6)和肿瘤坏死因子α(TNF-α)的影响,探讨决明子治疗高脂血症的调脂外作用机制。方法:采用饲喂高脂饲料和饮用10%乙醇溶液的方法建立高脂血症大鼠模型。造模成功后,随机分为5组,模型组,阳性对照组(血脂康,0.15 g·kg-1),决明子低、中、高(4,8,16 g·kg-1)剂量组,各组均按10 mL·kg-1体积ig给予相应药物,并设正常对照组,连续ig 4周。灌胃给药期间继续饲喂高脂饲料和饮用10%乙醇溶液。末次给药后,采血,用全自动生化仪测定大鼠血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白-胆固醇(LDL-C)、高密度脂蛋白-胆固醇(HDL-C)、葡萄糖(Glu)的含量,并用ELISA法测定血清中IL-6和TNF-α的含量。结果:与正常对照组比较,高脂血症模型组大鼠血清TC,TG,LDL-C,Glu含量显著升高(P<0.01),HDL-C显著降低(P<0.01);IL-6和TNF-α含量显著升高(P<0.01)。与模型组比较,决明子乙醇提取物高、中剂量组能显著降低大鼠血清中TC,TG,LDL-C,Glu的含量,显著升高HDL-C的含量(P<0.01);显著降低大鼠血清IL-6和TNF-α水平(P<0.01)。结论:决明子乙醇提取物不仅可有效改善高脂血症模型大鼠的血糖及血脂水平,亦可明显降低炎性细胞因子IL-6和TNF-α水平,这可能有利于动脉粥样硬化斑块的稳定。Objective： To observe effects of ethanol extraction from Cassiae Semen on serum interleukin-6（IL-6） and tumour necrosis factor-α （TNF-α） in hyperlipidemia rats. Method： The rat hyperlipidemia model was established by feeding high calorie-diet and 10% alcohol. After modeling, the selected rats were randomly divided into six groups：normal control group, model group, positive control group, low dose group, medium dose group and high dose group. Positive control group was administrated Xuezhikang capsule at dose of 0.15 g·kg^-1. Low dose group, medium dose group and high dose group were administrated at different concentrations of ethanol extraction from Cassiae Semen, which was respectively 4,8,16 g·kg^-1. All groups were applied by intragastric administration by 10 mL·kg^-1 for 4 weeks. Except normal control group, the remains were fed with high calorie-diet and 10% alcohol. After the last administration, the serum content of total cholesterol （TC）, triglyceride（TG）, low-density lipoprotein-cholesterol（LDL-C）, high-density lipoprotein-cholesterol（HDL-C） and glucose（Glu） were detected by automated biochemistry analyzer. And the content of IL-6 and TNF-α was determined by ELISA. Result： The content of TC, TG, LDL-C, Glu, IL-6 and TNF-α was increased, and the content of HDL-C was decreased in model group, compared with normal control group （P〈0.01）.The content of TC, TG, LDL-C and Glu was decreased, and the content of HDL-C was increased in medium dose group and high dose group of ethanol extraction from Cassiae Semen, compared with model group （P〈0.01）. At the same time, the content of IL-6 and TNF-α was decreased in medium dose group and high dose group （P〈0.01）. Conclusion： Ethanol extraction from Cassiae Semen can regulate the metabolic process of blood lipids and blood glucose levels of hyperlipidemia model rats, also can decrease the content of IL-6 and TNF-α in serum.

关 键 词：决明子 高脂血症 白介素6 肿瘤坏死因子α 

分 类 号：R285.5[医药卫生—中药学]