微波消解-分子荧光猝灭法测定蜂胶中痕量硒  

Microwave digestion- molecular fluorescence quenching method for the determination of trace selenium in propolis

作　　者：张修景[1] 

机构地区：[1]菏泽学院化学与化工系,山东菏泽274015

出　　处：《食品与发酵工业》2014年第3期208-210,共3页Food and Fermentation Industries

基　　金：山东省重点学科应用化学资助项目(No:2011350-1)

摘　　要：建立了微波消解．分子荧光猝灭法测定蜂胶中痕量硒的方法。蜂胶样品经微波消解后，用HCI将消化液中的Se（VI）全部还原成se（IV），再与碘化物和罗丹明B发生显色反应；当I3-与罗丹明B形成离子缔合物时，罗丹明B溶液的荧光猝灭，硒浓度在0～0．100ug／mL线性范围内荧光猝灭值与Se（iv）的浓度成正比。其线性回归方程是，，=3536．1x＋4．76，线性相关系数为0．9995，加标回收率在96．8％-103％，相对标准偏差（n=4）〈4．2％，检测限为0．0010ug／mL。A method is established to determine trace selenium in propolis by microwave digestion-molecular fluorescence quenching. After microwave digestion, propolis samples reduces Se（ VI ） in digestion solution to Se（ IV ） with hydrochloric acid. Then, the propolis samples make chromogenic reaction with iodide and Rhodamine B. When I3 - and Luo Danming B form an ion association complex, the fluorescence of Luo Danming B solution is suddenly extinguished. When the selenium concentration is in the linear range of 0- 0. 100 ug/mL, the fluorescence quenching is proportional to the concentration of selenium （IV）. The linear regression equation is y = 3 536. 1x ＋ 4. 761 9. The linear correlation coefficient is 0. 9995, and the recovery rate is between 96.8% to 103%. The relative standard deviation（n =4）is less than 4.2%. The limit of detection is 0.001 0ug/mL. The method has high efficiency, less selenium evaporating, accurate measurement and strong practicability.

关 键 词：蜂胶 微波消解 猝灭 硒 罗丹明B 

分 类 号：O657.3[理学—分析化学] S896.6[理学—化学]