FGF21对大鼠肝癌模型中TGF-β信号通路的影响  

Effect of FGF21 on TGF-β Signaling Pathway in Rat Liver Cancer

作　　者：江松敏 陈智心 蔡琳[2] 

机构地区：[1]瑞安市人民医院,浙江瑞安325200 [2]温州医科大学药学院,浙江温州325035

出　　处：《中国现代应用药学》2014年第3期261-265,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省教育厅科研项目(Y200907097);温州市科技计划项目(Y20090257)

摘　　要：目的研究FGF21通过TGF-β/Smads信号通路延缓肝癌发生发展的分子机制。方法采用Western blot法检测各组大鼠肝癌组织的TGF-β1,TGF-βRⅡ,Smad 2,3,4,7的蛋白表达水平,采用Q-PCR法检测各组大鼠肝癌组织的TGF-β1,TGF-βRⅡ,Smad 2,3,4,7的mRNA表达水平。结果在不同FGF21浓度下,TGF-β1,TGF-βRⅡ,Smad 2,3,4,7表达水平不同(P<0.05)。结论 FGF21通过促进TGF-β/Smads信号通路中的信号分子或受体的表达,或者通过下调此信号通路的阻断分子而抑制肿瘤发生。OBJECTIVE To research the possible molecular mechanisms of FGF21 via TGF-β/Smads signal pathway to delay the development of liver cancer. METHODS Detect the protein expressions of TGF-β1, TGF-β R Ⅱ, Smad 2,3,4,7 ofrespective rats cancer tissue with Western blot and detect the mRNA expression of TGF-β1, TGF-β R Ⅱ, Smad 2,3,4,7 of respective rats cancer tissue with Q-PCR. RESULTS The expressions of TGF-β1, TGF-β R Ⅱ, Smad 2,3,4,7 varied with the different concentrations of FGF21（P〈0,05）. CONCLUSION By promoting TGF-β/Smads signaling pathway in the expression of signaling molecules or receptors, or by decreasing the blocking of this signaling pathway, tumor was inhibited by FGF21.

关 键 词：FGF21 TGF-Β1 SMAD 

分 类 号：R965.2[医药卫生—药理学]