金雀异黄素对K562细胞的生长抑制和诱导凋亡作用  

Growth Inhibition and Apoptosis Induction Effects of Genistein on K562 Cells

作　　者：刘润[1] 高晓兰[1] 王茂源[1] 王强[1] 秦冠华[1] 

机构地区：[1]甘肃中医学院,兰州730000

出　　处：《中国现代应用药学》2014年第3期287-289,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的探讨金雀异黄素对人红白血病细胞系K562细胞的生长抑制、诱导凋亡作用。方法不同浓度的金雀异黄素处理K562细胞后,用MTT比色法检测细胞生长增殖作用,Wright-Gimesa法观察细胞的形态学变化,FCM检测K562细胞凋亡峰。结果金雀异黄素对K562细胞具有与时间、浓度呈正相关的生长抑制作用。形态学显示金雀异黄素高浓度组细胞发生核固缩、核碎裂,并有凋亡小体出现。细胞周期显示对照组K562 G2/M期细胞8.9%,金雀异黄素75·mol·L·1时升高到60.6%(P<0.05);金雀异黄素75·mol·L-1时K562细胞亚G1期细胞为27.3%,对照组为0.4%(P<0.05)。结论金雀异黄素对K562细胞具有很强的生长抑制作用,K562细胞可发生明显G2/M期阻滞,金雀异黄素诱导了K562细胞发生凋亡。OBJECTIVE To study the growth inhibition and apoptosis induction effects of genistein on human leukemia cell. METHODS Growth inhibition effect of genistein on K562 cells was investigated by MTT colorimetry. Morphologic variations of genistein on K562 cells was detected by Wright-Gimesa. Genistein induced apoptosis of K562 cells was detected by flow cytometry. RESULTS Genistein could powerfully inhibit cell growth of K562 cells line. Morphology showed high genistein concentration cell nuclear apoptosis bodies appeared. Cell cycle analysis showed that the highly concertration genistein induced the growth inhibition and apoptosis on K562 cells（P〈0.05）. CONCLUSION Genistein make a much sensitive reactionon K562 cells. K562 cells can be significantly inhibited G2/M phase arrest, genistein induces apoptosis on K562 cells.

关 键 词：金雀异黄素 K562细胞 生长抑制 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]