双波长HPLC测定青阳参中青阳参苷元和告达庭的含量  

Determination of Cynanchogenin and Caudatin in Cynanchum Otophyllum Scheid by HPLC under Double UV Wavelengths

作　　者：张琳[1] 李晓誉[1] 徐世芳[1] 陈峰阳[1] 叶益萍[1] 

机构地区：[1]浙江省医学科学院,杭州310013

出　　处：《中国现代应用药学》2014年第3期317-319,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省科技计划项目(2011F20013);国家自然科学基金(21272213);浙江省医学重点学科群(XKQ-010-001)

摘　　要：目的建立采用双波长HPLC同时测定青阳参中青阳参苷元和告达庭含量的方法。方法采用高效液相色谱法，ZORBAXSB-C18（4．6mm×150mm，5gm）色谱柱，柱温40℃，流动相为乙腈-水，梯度洗脱，流速为1．0mL·min^-1，检测波长为220，260nm。结果青阳参苷元和告达庭均在2-64Pg·mL^-1内呈良好的线性关系，r均为1；平均回收率为100．1％和97．9％，RSD值分别为1．34％和1.37％（n=6）。结论该方法快捷简单，精密可靠，重复性好，可为青阳参及其制剂的质量控制提供参考。OBJECTIVE To estabish an HPLC method under double UV wavelengths for determination of cynanchogenin and caudatin in Cynanchum otophyllum Scheid. METHODS ZORBAX SB-C18 column （4.6 mm× 150 mm, 5 μm） was used and the temperature of column was 40 ℃. The mobile phase consisted of methanol-acetonitrile with gradient elution. The flow rate was 1.0 mL·min^-1. The detection wavelength was set at 220 and 260 nm. RESULTS The cynanchogenin and caudatin had a good linearity relationship in 2 μg·mL^-1 with r=1. The average recoveries were 100.1% and 97.9% with RSD of 1.34% and 1.37%（n=6）. CONCLUSION The method is simple, rapid, accurate and provides the reference for the quality identification of Cynanchum otophyllum Scheid and its preparation.

关 键 词：高效液相色谱法 青阳参 青阳参苷元 告达庭 

分 类 号：R917.101[医药卫生—药物分析学]