突触蛋白Synapsin在氧糖剥夺模型中的表达及意义  

The expression and significance of Synapsin protein in OGD model

作　　者：刘洪雨[1] 莽靖[1] 杨乐[2] 胥桂华[1] 李宗树[2] 王娇琦[1] 何金婷[1] 徐忠信[1] 

机构地区：[1]吉林大学中日联谊医院,吉林长春130033 [2]吉林省人民医院,吉林长春130021

出　　处：《中国实验诊断学》2014年第3期349-351,共3页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然基金面上项目(81371298);吉林省科技发展计划国际合作项目(20120723);吉林省产业技术研究与开发项目(2013C030-4);吉林省科技发展计划青年基金项目(201201016/20130522024JH)

摘　　要：目的探讨Synapsin蛋白在氧糖剥夺模型中的表达变化及意义。方法 PC12细胞进行氧糖剥夺处理,建立缺血性脑损伤模型,Western blot方法检测氧糖剥夺模型细胞中Synapsin蛋白的表达。结果应用终浓度50ng/ml NGF的DMEM完全培养基培养PC12细胞,对PC12细胞进行氧糖剥夺处理,与对照组相比,氧糖剥夺(OGD)时间越长,Synapsin蛋白表达水平越低,蛋白表达的条带越淡。结论 Synapsin蛋白对PC12细胞的突出增长有促增殖作用,随氧糖剥夺时间的延长,神经细胞的神经元特性、突触功能逐渐减低。Objective To investigate the expression and significance of Synapsin protein in OGD model.Methods established ischemic brain injury model in PC12 cells OGD treatment,western blotting was used to detect the expres sion of Synapsin protein in OGD model cells.Results applying a final concentration of 50 ng/ml NGF in DMEM,PC12 cells against oxygen-glucose deprivation treatment,compared with the control group,with the OGD time increases intracellular protein Synapsin gradually reduced,strip protein expression was significantly lighter.Conclusion Synapsin protein on the growth of PC12 cells have prominent proliferation effect,with the OGD time,the characteristics of the nerve cells of neurons,synaptic function gradually reduced.

关 键 词：Synapsin蛋白 PC12细胞 氧糖剥夺模型 

分 类 号：R341[医药卫生—基础医学]