血清稀释与否对检测乙型肝炎核心抗体结果的影响  

Effect on Detection of Hepatitis B Core Antibody Whether Serum Dilution or Not

作　　者：张碧莹 邢延芳[2] 王珍珠 耿丹 

机构地区：[1]铜川市人民医院检验科,陕西铜川727000 [2]延安大学附属医院检验科,陕西延安716000

出　　处：《中国实验诊断学》2014年第3期428-430,共3页Chinese Journal of Laboratory Diagnosis

基　　金：铜川市2013年科技惠民项目(KJ-2013-05)

摘　　要：目的探讨血清稀释与否对乙型肝炎核心抗体检测结果的影响。方法 ELISA作为初步检测法,配合电化学发光法检测乙肝五项和FQ-PCR检测HBV-DNA。结果稀释血清与原倍血清在检测乙型肝炎核心抗体的结果中存在不同。原倍血清HBcAb呈阳性,而稀释血清HBcAb呈阴性的有64例,占总标本的4.0%。经FQ-PCR检测稀释血清发现,其中2例标本HBV-DNA测定值>1.00×103copies/mL,判定为漏检,故ELISA稀释法检测HBcAb漏检率为3.1%。结论 ELISA稀释法检测低浓度的HBcAb,血清因稀释易呈假阴性,需根据临床需要结合FQPCR法检测HBV-DNA判断乙肝病毒的复制情况。Objective To investigate the effects on detection of hepatitis B core antibody whether serum dilution or not.Methods ELISA assay as a preliminary,confirmed HB five by chemiluminescence detection and HBV-DNA by FQ-PCR detection.Results There were differents between original serum and dilution serum in detecting hepatitis B core antibody.There were 64 cases which HBcAb of original sersum was positive but dilution serum was negative,4.0％ of the total sample.Measured by FQ-PCR was found,dilution serum including two cases of HBV-DNA samples measured values＞1.00× 103copies/mL,judged undetected.So missing rate of HBcAb in diluted serum was 3.1％ by ELISA assay.Conclusion ELISA assay HBcAb,due to the presence of a small amount of serum dilution and false negative,further testing confirmed to be combined with FQ-PCR assay to determine HBV-DNA replication of hepatitis B virus.

关 键 词：乙肝病毒 核心抗体 稀释 HBV-DNA 

分 类 号：R512.62[医药卫生—内科学]