果蝇P-TEFb与人源P-TEFb组成亚基间相互作用的研究  

Interactions between drosophila P-TEFb and human P-TEFb subunits

作　　者：陈斌[1] 吴传芳[1] 欧阳劲 秦岭[1,3] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]成都南诺格生物科技有限责任公司,成都610064 [3]成都华高瑞甜科技有限公司,成都610064

出　　处：《四川大学学报（自然科学版）》2014年第2期396-401,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(30970625)

摘　　要：本实验克隆了果蝇与人源P-TEFb(positive transcription elongation factor)组成亚基CycT、CDK9C末端分别带有Flag、HA标签的真核表达质粒,并在293细胞中验证了克隆所得质粒的有效表达.使用免疫共沉淀方法证实了两个种属P-TEFb组成亚基间结构的相似性;借助HIV-luciferase与Rellina的双荧光报告系统证实了两个种属P-TEFb组成亚基间功能上的相似性.In this study eukaryotic expression plasmid of Drosophila and human P-TEFb＇s subunit gene CDK9 and CycT was cloned,which each of the subunit has a Flag or HA tag in the C terminal respec-tively.Also was proved well express in the 2 9 3 Cell line.With the method of immunoprecipitation Dro-sophila P-TEFb and human P-TEFb subunits＇structure similarity was well confirmed.By means of dual-luciferase report system of HIV-luciferase and Rellina,P-TEFb subunits＇function similarity of the two species was also well affirmed.

关 键 词：P-TEFB CDK9 CycT FLAG HA 免疫共沉淀 双荧光报告系统 

分 类 号：Q78[生物学—分子生物学]