拟南芥AtDWD与RCAR1相互作用的初步研究  

Preliminary study of proteins interacting with RCAR1 and AtDWD in Arabidopsis thaliana

作　　者：姜欣[1] 李德款[1] 陶志文[1] 袁德志[1] 杨毅[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2014年第2期402-407,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(31171586);国家转基因重大专项(2011ZX08009-003-002)

摘　　要：采用pull down和酵母共转化的方法研究了AtDWD与RCAR1之间的相互作用。体外pull down试验得出 AtDWD与 RCAR1之间存在明显的相互作用；酵母共转化试验显示AtDWD的截断区域中只有 WD45和 WD345转化子可以在 SD 四缺培养基平板上长出正常的酵母菌落。实验结果表明,AtDWD C端的145~350位氨基酸序列中含有与 RCAR1相互作用的功能域。Pull down and yeast co-transformation methods were used to study the interaction between At-DWD and RCAR1 in research.The significant interaction of them was tested by pull down in vitro.On-ly the truncated area AtDWD WD45 and WD345 tansformants could grow into normal yeast colonies on SD-deficient medium plate,this result was studied by yeast co-transformation.It is confirmed that the interacting area of AtDWD with RCAR1 located at 145~350 aa of AtDWD C terminus.

关 键 词：酵母双杂交 Pulldown RCAR1 AtDWD 

分 类 号：Q78[生物学—分子生物学]