龙血竭HPLC指纹图谱研究  

Study on HPLC Fingerprint of Dracaena Cochinchinensis

作　　者：徐玉玲[1] 梁慧[2] 凌娅[2] 杜思雯[1] 伍利华[3] 苟小军[4] 

机构地区：[1]成都大学实验技术中心,四川成都610106 [2]江苏康缘药业股份有限公司,江苏连云港222001 [3]广西中医药大学药学院,广西南宁530001 [4]成都大学生物产业学院,四川成都610106

出　　处：《成都大学学报（自然科学版）》2014年第1期1-3,共3页Journal of Chengdu University（Natural Science Edition）

摘　　要：为建立龙血竭指纹图谱质量控制方法,采用HPLC法测定,液相色谱仪Agilent 1100,色谱柱为Waters Xterra C184.6×250 mm,5μm;流动相为0.1%的磷酸溶液和乙腈梯度洗脱,流速为0.7 mL/min,检测波长为280 nm,理论板数按参照物(龙血素B)峰计算不低于6000.实验结果表明,10批成品指纹图谱与对照指纹图谱计算相似度,其结果均大于0.90.该方法稳定可行,可用于龙血竭质量控制.To establish fingerprint quality control method of Dracaena cochinchinensis, this paper adopts HPLC method for determination. Agilent I100 liquid chromatography and the Waters Xterra Cls （4.6 mm × 250 mm, 5 tma）are used. The mobile phase is a linear gradient of acetonitrile and 0.1% Oahophosphoric acid at the flow rate of 0.7 mL· min- 1. UV detection wavelength is set at 280 nm. The number of theoreti- cal plates is not less than 6 000 according to reference compound （Loureirin B） peak. The experimental re- suits show that the overall similarity is greater than 0.9 by comparing the fingerprints of 10 batches of sam- pies and the representative f＇mgerprints. We conclude that this method is stable and feasible, which can be used for research and quality control of Dracaena cochinchinensis.

关 键 词：龙血竭 HPLC 指纹图谱 相似度 质量控制 

分 类 号：R282.5[医药卫生—中药学]