黄柄曲霉ASD菌株抑菌谱测定及抑菌物质初探  

Inhibition Spectrum Determination of Aspergillus flavipes ASD Strains and Primary Studies on Its Antibacterial Substance

作　　者：谢小平[1] 刘长远[2] 赵奎华[2] 王辉[2] 关天舒[2] 梁春浩[2] 贾姝[1] 张里[1] 

机构地区：[1]沈阳农业大学植物保护学院,辽宁沈阳110866 [2]辽宁省农业科学院植物保护研究所,辽宁沈阳110161

出　　处：《中国蔬菜》2014年第4期34-37,共4页China Vegetables

基　　金：辽宁省科技攻关项目(2011214002);辽宁省设施蔬菜产业创新团队项目(2010015)

摘　　要：采用对峙培养法对黄柄曲霉(Aspergillus flavipes)ASD菌株抑菌谱进行了测定,试验结果表明:黄柄曲霉ASD菌株具有较广的抑菌谱,对辣椒疫病菌(Phytophthora capsiciLeonian)、番茄晚疫病菌(Phytophthora infestans)有较好的抑制作用。ASD菌株发酵液对辣椒疫病菌的抑菌带宽度为7.4mm,抑菌活性较强。ASD菌株的发酵液经50%硫酸铵饱和度沉淀并透析后得到的抑菌物质对辣椒疫病菌的抑菌效果最好,抑菌活性可达80.78%,且抑菌物质为蛋白类物质。The inhibition spectram of Aspergillusflavipes ASD strains was measured by plate confrontation test. The results showed that ASD strains had a wide-range inhibition. It had better inhibitory effect on Phytoph- thora capsici and Phytophthora infestans. The results showed that the inhibitory effect of broth of ASD strains was pretty good on Phytophthora capsici. Its inhibitory diameter was 7.4 mm. After 50% （ NH4 ） 2SO4 saturation precipitation of broth of ASD strains and dialysis, the antibacterial substance was obtained which showed the best inhibitory effect on Phytophthora capsici. The inhibition effect could reach 80.78%, and moreover the antibacterial substance was albuminoidal substances.

关 键 词：黄柄曲霉ASD菌株 抑菌谱 抑菌物质 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]