The Development of A Fluorescence Polarization Immunoassay for Aflatoxin Detection  

作　　者：SHENG Ya Jie EREMIN Sergei MI Tie Jun ZHANG Su Xia SHEN Jian Zhong WANG Zhan Hui 

机构地区：[1]Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety [2]Department of Chemistry, M.V. Lomonosov Moscow State University

出　　处：《Biomedical and Environmental Sciences》2014年第2期126-129,共4页生物医学与环境科学（英文版）

基　　金：supported by grants from the International Science&Technology Cooperation Program of China(2009DFA32330);the Special Fund for Agro-scientific Research in the Public Interest(No.201203040)

摘　　要：A fluorescence polarization immunoassay （FPIA） was developed for the analysis ofaflatoxins （AFs） using an anti-aflatoxin B1 （AFB1） monoclonal antibody and a novel fluorescein-labeled AFB1 tracer. The FPIA showed an IC50 value of 23.33 ng/mL with a limit of detection of 13.12 ng/mL for AFB1. The cross-reactivities of AFB1, AFB2, AFG1, AFG2, AFM1, and AFM2 with the antibody were 100%, 65.7%, 143%, 23.5%, 111.4%, and 2%, respectively. The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs, albeit not AFG2 and AFM2. The total time required for analyzing 96 samples in one microplate was less than 5 rain. This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs.A fluorescence polarization immunoassay （FPIA） was developed for the analysis ofaflatoxins （AFs） using an anti-aflatoxin B1 （AFB1） monoclonal antibody and a novel fluorescein-labeled AFB1 tracer. The FPIA showed an IC50 value of 23.33 ng/mL with a limit of detection of 13.12 ng/mL for AFB1. The cross-reactivities of AFB1, AFB2, AFG1, AFG2, AFM1, and AFM2 with the antibody were 100%, 65.7%, 143%, 23.5%, 111.4%, and 2%, respectively. The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs, albeit not AFG2 and AFM2. The total time required for analyzing 96 samples in one microplate was less than 5 rain. This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs.

关 键 词：FPIA AFB The Development of A Fluorescence Polarization Immunoassay for Aflatoxin Detection AFM EDF 

分 类 号：R114[医药卫生—卫生毒理学]