苏州市学龄前儿童全血锰的测定及其参考范围建立  

Determination of whole blood manganese of pre-school age children in Suzhou and establishment of the reference range

作　　者：石慧[1] 马扣祥[2] 顾炳仁 许奇[3] 石冬敏[4] 马钧[4] 

机构地区：[1]苏州卫生职业技术学院,江苏苏州215009 [2]苏州大学,江苏苏州215006 [3]苏州市药品检验所,江苏苏州215103 [4]江苏南京医科大学苏州市立医院本部,江苏苏州215002

出　　处：《现代预防医学》2014年第7期1201-1202,1206,共3页Modern Preventive Medicine

基　　金：苏州市科技计划项目(sys201162)

摘　　要：目的 建立苏州市区学龄前儿童全血锰（Mn）的参考范围。方法 全血样经微波消解处理后，用等离子发射光谱法（ICP-AES）测定，参照NCCLS发布的临床实验室（C28-A2）的文件对儿童血锰含量数据进行统计处理，确定参考范围。结果 本方法的批内CVw为1.6%～2.1%，批间CVb为2.0%～2.4%，偏倚CVbias为0.52%～0.68%，相对扩展不确定度为5.23%～6.52%，加标回收率在96.7%～98.1%之间。苏州市区学龄前儿童全血锰的参考范围为4.72～11.45 μg/L（男：4.76～11.51 μg/L，女：4.70～11.42 μg/L）。锰元素参考范围在性别间的差异无统计学意义（P＞0.05）。结论 等离子发射光谱法测定全血锰，方法简单、可靠，建立的全血锰参考范围符合苏州市学龄前儿童的人群特点，可在临床推广应用。Objective To establish the reference range of blood manganese （Mn） of pre-school age in Suzhou. Methods Samples were digested by microwave, and then determined by ICP-AES, statistical processing of data on children＇s blood manganese content were conducted by Clinical laboratory （C28-A2） file NCCLS released, and the reference range was defined. Results The within-run CVw was 1.6%-2.1%, the between-run CVb was 2.0%-2.4%, the bias was 0.52%-0.68% and the relative expanded uncertainty was 5.23%-6.52%. The standardized recovery rate was 96.7%-98.1%. The reference range of Mn was 4.72-11.45μg/L （male： 4.76-11.51μg/L, female： 4.70-11.42μg/L）. The reference range of Mn had no difference with sex （P〉0.05）. Conclusion This method is simple and reliable. The reference range in the study is agreed with the population characteristics of pre-sehool age children in Suzhou, and the analyzer can be applied in clinical usage.

关 键 词：学龄前儿童 全血锰 参考范围 

分 类 号：R174[医药卫生—妇幼卫生保健]