脂联素基因多态性与2型糖尿病的关联研究  

An association study between ADIPOQ gene polymorphisms and type 2 diabetes mellitus

作　　者：修良昌[1,2] 张杨[2] 林美华[2] 欧阳平[2] 覃继恒 赵小蕾[2] 孔丹莉[2] 饶绍奇[2] 丁元林[1,2] 

机构地区：[1]南方医科大学公共卫生与热带医学学院生物统计学系,广东广州510515 [2]广东医学院公共卫生学院流行病与卫生统计学教研室,广州东莞523808

出　　处：《现代预防医学》2014年第7期1262-1265,1269,共5页Modern Preventive Medicine

基　　金：国家自然科学基金(81273166);广东省自然科学基金(S2012010008271);东莞市科技计划项目(2012105102010);湛江市科技攻关计划项目(2012C3103012);广东医学院重点学科项目(XZ1105)

摘　　要：目的探讨广东汉族人群中脂联素基因(ADIPOQ)的单核苷酸多态性(SNP)与2型糖尿病的关联。方法采用SNPscanTM多重SNP分型试剂盒,对筛选的2个tagSNP(rs822396、rs3774261)在595名2型糖尿病患者和725名健康对照中进行基因分型,并应用χ2检验、Logistic回归模型等方法分析SNP位点与2型糖尿病的关联性。结果调整性别、BMI和甘油三酯等因素后,rs822396在等位基因关联分析和基因型关联分析中均无统计学意义(P>0.05),rs3774261在等位基因关联分析中亦无统计学意义[P=0.077,OR(95%CI)=1.18(0.98,1.41)],但在显性模型下的基因型关联分析中有统计学意义[P=0.028,OR(95%CI)=1.36(1.03,1.79)]。结论在广东汉族人群中,rs822396与2型糖尿病无关联,rs3774261与2型糖尿病的遗传易感性有关,但其具体机制尚需进一步探讨。Objective To evaluate the association between two single nucleotide polymorphisms located in ADIPOQ gene and type 2 diabetes mellitus in Han population in Guangdong Province. Methods Two tagSNPs （rs822396 and rs3774261） were picked out from ADIPOQ gene. Then SNP genotyping was performed using SNPscanTM Kit in two cohorts, including a total of 595 cases and 725 controls. Finally, Chi-square test, logistic regression model and other methods were employed in the following statistical analysis. Results No association between SNP rs822396 with T2DM was found in allelic association analysis and genotypic association analysis （P〉0.05） after adjustment for sex, BMI and TG, rs3774261 also had no association in allelic gene association analysis[P=-0.077, OR （95% CI） =1.18 （0.98, 1.41） ]. However, the GG＋GA genotype of SNP rs3774261 was associated with T2DM in dominant model [P=-0.028, OR （95% C/） =1.36 （1.03, 1.79） ]. Conclusion SNP rs822396 of ADIPOQ gene is not associated with T2DM. However, rs3774261 is associated with increased risk of T2DM in Han population in Guangdong Province, and additional studies are needed to delineate further the role of this common variant in the pathogenesis of T2DM.

关 键 词：脂联素 糖尿病 2型 多态性 单核苷酸 

分 类 号：R181.32[医药卫生—流行病学]