基于ISSR标记的少果毛一球悬铃木遗传稳定性分析  

Analysis on less fruit hair Platanus occidentalis genetic stability based on ISSR marker

作　　者：姚军 张丽芳[2] 涂炳坤[2] 丁昭全 

机构地区：[1]武汉市园林科学研究所,湖北武汉430081 [2]华中农业大学园艺林学学院园艺植物生物学教育部重点实验室,湖北武汉430070

出　　处：《中南林业科技大学学报》2014年第2期6-11,共6页Journal of Central South University of Forestry & Technology

基　　金：武汉市园林局科研项目(武园[2009]55号)

摘　　要：本研究应用ISSR分子标记,对3个不同立地条件下的不同嫁接年限的少果毛一球悬铃木优良品种‘园科115’一球悬铃木Platanus occidentalis‘Yuanke 115’的接穗（13 a、14 a与30 a）以及两个相应的砧木（二球悬铃木）进行遗传稳定性分析。16条引物在5个样品中共扩增出75条谱带,其中27条具有多态性,多态性比例为36%。根据ISSR标记数据,基于遗传相似系数的聚类分析显示：5个悬铃木样品间的遗传相似系数在0.680 0～1.000之间,遗传相似系数在0.77时,5个悬铃木样品归为一类;遗传相似系数在0.98时,3个不同嫁接年限的‘园科115’一球悬铃木接穗归为一类。其中,嫁接14 a与30 a的一球悬铃木接穗的遗传相似系数为1,它们与嫁接13 a的一球悬铃木接穗的遗传相似系数也高达0.986 7。以上结果说明：‘园科115’一球悬铃木在不同的立地条件下,经历不同的嫁接时间（最长达30 a）,依然具有高度的遗传稳定性。The analyses of genetic stability were performed on the 3 scions of Platanus occidentalis‘Yuanke 115’in different site conditions with different grafting time（13,14 and 30 years respectively） and 2 corresponding rootstocks based on ISSR molecular marker.There were 75 bands in total amplified with 16 ISSR primers in 5 samples,of which 27 bands were polymorphic,and the polymorphic percentage was 36%.According to ISSR markers data,the cluster analysis based on genetic similarity coefficients was conducted.The results are as follows： The genetic similarity coefficients between 5 samples ranged from 0.6800 to 1.0000;5 samples were grouped into one category with the genetic similarity coefficient of 0.77;The 3 scions were grouped into one category with the genetic similarity coefficient of 0.98;The genetic similarity coefficient between scions with 14 years and 30 years grafting time was 1.0000;Either of the scions with 14 years and 30 years grafting time had a genetic similarity coefficient of 0.9867 with the scion with 13 years grafting time.The results above show that P.occidentalis‘Yuanke 115’confers high level of genetic stability in different site conditions and through different period of grafting time（up to 30 years）.

关 键 词：'园科115’一球悬铃木 分子标记 遗传稳定性 ISSR分子标记 

分 类 号：S722.31[农业科学—林木遗传育种] S687.1[农业科学—林学]