固定化假丝酵母脂肪酶对光皮树油的吸附性能  

Adsorption kinetics of immoblized candida lipase to Swida wilsoniana oil

作　　者：肖志红[1,2] 吴红[2] 李昌珠[2] 张爱华[2] 刘汝宽[2] 吴晓芙[1] 

机构地区：[1]中南林业科技大学,湖南长沙410004 [2]湖南省林业科学院,湖南长沙410004

出　　处：《中南林业科技大学学报》2014年第2期117-121,共5页Journal of Central South University of Forestry & Technology

基　　金：国家林业局林业公益性行业科研专项(201004071);国家科技支撑计划(2011BAD22B04);湖南省自然科学基金(14JJ2141)

摘　　要：通过TEM、比表面孔分布测定仪等对含环氧基团的聚合物载体(GHD)性能进行了表征。以GHD为载体,制备了固定化假丝酵母脂肪酶。研究了光皮树油在固定化假丝酵母脂肪酶(I-CRL)上的吸附行为,从吸附平衡和吸附动力学角度探讨了吸附机理。结果表明,光皮树油在I-CRL上的等温吸附过程符合Langmiur方程,为单分子层吸附。实验数据能很好的拟合二级动力学吸附方程,当采用聚合物GHD(40)为载体,初始光皮树油浓度(Ce)为210 mg/g,在温度为325 K,330 K,335 K时,由Arrhenius公式求得的表观活化能Ea为30.44 kJ/mol,吸附过程为吸热反应。A polymeric carrier（GHD） supported with epoxy groups was characterized by TEM and Micromeritics ASAP 2010（a kind of specific surface pore distribution analyzer）.The immobilized candida lipase（I-CRL） was prepared with GHD as the carrier,further the adsorption performance of Swida wilsoniana oil on I-CRL was studied,and the adsorption mechanisms were investigated from the angles of adsorption equilibrium and kinetic.The results show that the isothermal adsorption of S.wilsoniana oil on I-CRL well agreed with that of Langmiur isothermal model,indicating that the adsorption of S.wilsoniana oil oil on I-CRL was a monolayer adsorption.The experimental data fitted well to the second-order kinetic model in the case that GHD（40） as carrier and the initial Ce was 210 mg/g.At the meantime,the apparent activation energy（Ea= 30.44 kJ/mol） can be calculated based on Arrhenius equation by the data obtained at 325 K,330 K and 335 K.The adsorption of Swida wilsoniana oil on I-CRL was proved to be an endothermic reaction.

关 键 词：光皮树油 固定化假丝酵母脂肪酶 多孔高分子微球 吸附平衡 吸附动力学 

分 类 号：S727.32[农业科学—林木遗传育种]