康复1号对小鼠成骨细胞增殖的影响  

Effect of Herb Rehabilitation No.1 on Mouse Osteoblast Proliferation

作　　者：赵德春 吴绍长 邢葆平[3] 李晓一[3] 

机构地区：[1]浙江省武义县妇幼保健院,武义322300 [2]浙江省丽水市第二人民医院 [3]浙江省立同德医院

出　　处：《浙江中西医结合杂志》2014年第3期198-199,共2页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省中医药科学研究基金项目(No.2012ZB004);浙江省医药卫生平台重点资助计划(No.2012ZDA007);浙江省医学卫生科学研究基金项目(No.2010KYB133)

摘　　要：目的观察康复1号提取物对小鼠成骨细胞MC3T3-E1细胞增殖的影响。方法将稀释成不同梯度浓度的康复1号提取物和成骨样细胞MC3T3-E1共同体外培养,采用MTT法检测细胞的增殖。结果康复1号提取物低浓度组(1:2000)对MC3T3-E1细胞增殖无影响(P>0.05);中浓度组(1:1000)对细胞增殖影响明显,增殖率48.1%(P<0.05);高浓度组(1:250)增殖率68.1%(P<0.01)。结论体外实验表明,康复1号能明显促进小鼠MC3T3-E1细胞的增殖。Objective To observe the effect of rehabilitation No.1 herb extracts on the proliferation of MC3T3-E1 cells. Methods Osteoblast-like MC3T3-E1 cells were cultured in vitro with different concentrations of herb extracts of rehabilitation No.1. Cell proliferation was detected by using MTT method. Results Low concentration group（1：2000） of the rehabilitation No.1 herb extract had no effect on the proliferation of MC3T3-E1 cells; the proliferation rate was 48.1% in middle concentration group （1：1000）（P＆lt;0.05）; and the proliferation rate of high concentration group （1：250） was 68.1%（P＆lt;0.01）. Conclusion Rrehabilitation No.1 herb extracts can promote the proliferation of MC3T3-E1 cells in vitro.

关 键 词：小鼠 骨质疏松症 MC3T3-E1细胞 康复1号 

分 类 号：R580[医药卫生—内分泌]