盐胁迫对垂穗披碱草幼苗生理指标的影响  

Effect of salt stress on the physiological characteristics of Elymus nutans

作　　者：杨月娟[1,2] 周华坤[1] 王文颖[3] 殷恒霞[3] 姚步青[1] 余欣超 赵新全[1] 王发刚 

机构地区：[1]中国科学院西北高原生物研究所,西宁8100011 [2]中国科学院大学,北京100049 [3]青海师范大学生命与地理科学学院,西宁810008 [4]青海省畜牧兽医职业技术学院,青海湟源812000

出　　处：《兰州大学学报（自然科学版）》2014年第1期101-106,共6页Journal of Lanzhou University(Natural Sciences)

基　　金：国家自然科学基金项目(31172247;41030105;31260127);国家自然科学基金青年基金项目(31201836);中国科学院战略性先导科技专项子课题项目(XDA05070202);国家科技支撑课题专题项目(2011BAC09B06-02);青海省重点实验室发展专项资金计划项目(2013-Z-Y07);青海省科技厅国际合作项目(2010-H-809)

摘　　要：采用不同浓度NaCl溶液(0,50,150,250 mmol/L)处理萌发的垂穗披碱草幼苗,通过检测垂穗披碱草在盐胁迫逆境下生理生化的变化,并与老芒麦进行比较,研究其幼苗对盐胁迫的反馈机制,为垂穗披碱草在盐碱地的建植和恢复改良提供参考.结果表明:垂穗披碱草叶片可溶性糖质量分数和过氧化氢酶活性变化一致,随着盐浓度的增加呈现先上升后下降的趋势,50 mmol/LNaCl是其幼苗可溶性糖质量分数和过氧化氢酶活性的一个转折临界点;相对含水量、脯氨酸质量分数和超氧化物歧化酶活性变化一致,随着盐浓度的增加呈现上升趋势,分别在150,250 mmol/L盐浓度胁迫下,垂穗披碱草幼苗相对含水量、脯氨酸质量分数和超氧化物歧化酶活性显著高于其他材料的.方差分析结果表明盐胁迫对垂穗披碱草、老芒麦的生理指标影响显著.经相关分析筛选出对抗盐性影响显著的指标为相对含水量、可溶性糖质量分数、脯氨酸质量分数、超氧化物歧化酶、过氧化氢酶活性.经隶属函数法计算得出,垂穗披碱草具有一定的抗盐性,而且较老芒麦的强.Compared with those of Elymus sibiricus, the growth parameters of Elymus nutans at the early seedling stage were measured under four NaCl solutions with different concentrations （0, 50, 150, 250 mmol/L） and salt treatment was performed to explore and utilize the germplasm with high salt-tolerance and to provide physiological indicators for identifying salt-tolerance. It was found that the soluble sugar content and the activities of CAT were increasing at first and then declining with prolonged salt stress; a critical turning point occurred under the salt stress at 150 mmol/L, the relative water content, the proline content and the activities of SOD increased continually with NaCl stress concentration being increased, and accessions were higher than the others when the salt concentration was at 150 mmol/L and 250 mmol/L. Statistics analysis showed that the effects were different for E. nutans and E. sibiricus under salt stress. Correlation analysis indicated that the indices which had significant impact on salt resistance were the relative water content, soluble sugar content, proline content, and the activities of SOD and CAT. Calculated by the membership function, the salt-resistant ability of E. nutans was better than that of E. sibiricus.

关 键 词：垂穗披碱草 耐盐性 脯氨酸 可溶性糖 抗氧化酶 

分 类 号：Q945[生物学—植物学]