濒危植物观光木遗传多样性及遗传结构分析  

Genetic Diversity and Population Structure of an Endangered Species:Tsoongiodendron odorum Chun

作　　者：徐刚标[1] 吴雪琴[1] 蒋桂雄[2] 胡尚力[1] 刘雄盛[1] 肖玉菲[1] 郝博搏[1] 

机构地区：[1]中南林业科技大学林木遗传育种实验室,长沙410004 [2]广西壮族自治区林业厅,南宁530028

出　　处：《植物遗传资源学报》2014年第2期255-261,共7页Journal of Plant Genetic Resources

基　　金：国家林业公益性行业科研专项(201104033)

摘　　要：采用筛选的8条ISSR引物对濒危植物观光木13个野生种群的遗传多样性及遗传结构进行分析,结果显示:物种和种群水平上多态性位点百分率分别为79.67%和46.84%,Shannon表型多样性指数分别为0.3880和0.2192,与木兰科其他近缘植物相比,遗传多样性水平较高;分子方差分析表明,观光木野生种群间遗传分化系数为0.3752,种群间存在显著的遗传距离;STRUCTURE分析和UPGMA聚类表明,参试的13个野生种群可分为3大类群;相关性分析表明,种群间遗传距离与其地理距离呈显著正相关。南昆山、笔架山、弄相山种群的遗传多样性最丰富,建议优先加以保护。Tsoongiodendron odorum is an evergreen broad-leaved tree and at risk for becoming extinct in the wild. In this study, 13 T. odorum populations was analyzed using inter-simple sequence repeat （ISSR） markers to investigate the genetic diversity and structure. Eight primers produced discernible markers were applied. The percentage of polymorphic bands at the species and population level were 79.67% and 46.84%, respectively. Shannon’s phenotypic diversity index at the species and population level were 0.3880 and 0.2192, respectively. It indicates that T. odorum had a slightly higher level of genetic diversity than other related plants of the Magnoliaceae. The coefficient of genetic differentiation （ΦST） was 0.3752 by analysis of molecular variance （AMOVA） indicating that differentiation between populations was significant. Analysis of the ISSR data using STRUCTURE program and UPGMA revealed that the populations were clustered into three four population genetic clusters. The result of correlation analysis showed genetic distance was significantly positively correlated with geographical distance （DF=12，r=0.6635，p＜0.05）. The Nankunshan, Bijiasha and Nongxiangshan populations should be given priority for conservation, because these populations had the highest genetic diversity among all the 13 populations investigated.

关 键 词：观光木 ISSR标记 遗传多样性 遗传结构 

分 类 号：Q943[生物学—植物学]