中国黄瓜主栽品种SSR遗传多样性分析及指纹图谱构建  

Genetic Diversity Analysis and Fingerprinting Construction of Chinese Cucumber Main Cultivars by Simple Sequence Repeat Polymorphisms

作　　者：苗晗[1] 张圣平[1] 顾兴芳[1] 王烨[1] 陈磊[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《植物遗传资源学报》2014年第2期333-341,共9页Journal of Plant Genetic Resources

基　　金：公益性行业(农业)科研专项经费项目(200903008);农业部园艺作物生物学与种质创制重点实验室资助项目;国家现代农业产业技术体系建设专项(CRS-25)

摘　　要：应用简单重复序列（SSR）标记对从国内主要黄瓜育种单位征集到116份生产上主栽品种进行遗传多样性分析。结果表明35对引物共产生86个等位基因，每对引物平均扩增出2.46个等位基因，其中有效等位基因占70.99%，平均Shannon’s信息指数为0.639，平均PIC为0.382。116个品种的遗传相似系数（GS），分布在0.5029～0.9797之间。聚类分析结果表明在遗传距离0.25处可将供试材料分为2大类群。第1类共106个品种，可分为5个亚族主要包括华北密刺型、华南型、日本少刺型这三大类；第2类包含10个欧洲温室型品种。Genetic diversity of 116 cucumber（Cucumis sativus L.）main cultivars from different regions was estimated using simple sequence repeat （SSR） markers. Three hundred and fifty five polymorphic bands were detected by 35 pairs of SSR primers. Each SSR loci can amplify2.46 alleles on the average. 70.99% of the total alleles were effective for genetic diversity analysis. The average Shannon＇s information index and PIC is 0.639 and 0.382, respectively. The genetic similarity （GS） varied from 0.5029 to 0.9797 among the 116 cultivars. These cultivars were divided into two groups at the genetic distance of 0.25 by cluster analysis. The first group consisted of 106 cultivars belonged to three ecotypes, north China type, south China type and Japanese type. And this group can be divided into five subfamilies. Ten cultivars belonged to European greenhouse type were in the second group.

关 键 词：黄瓜 SSR标记 遗传多样性 聚类分析 指纹图谱 

分 类 号：S642.2[农业科学—蔬菜学]