体外流体剪切力作用下的骨髓间充质干细胞与树突状细胞的相互粘附作用观察  

作　　者：吴伟华[1] 李强[1] 滕小梅[1] 

机构地区：[1]苏州大学附属第一医院检验科,215006

出　　处：《浙江临床医学》2014年第3期348-349,共2页Zhejiang Clinical Medical Journal

摘　　要：目的：建立一种新方法来观察细胞之间的相互粘附作用。方法利用BioFlux数控剪切流条件下活细胞功能分析自动化系统，首先将C57骨髓间充质干细胞（C57-BMSC）、CD45＋CD31＋BMSC和CD45-CD31-BMSC三种细胞在微流体通道上包被，用Calcein-AM对DC细胞进行染色，将染色的细胞在特定剪切力下（0.5dynes/cm2）引入小室内，通过荧光倒置显微镜和BioFlux软件系统进行图像捕捉和数据分析。结果在荧光倒置显微镜下观察和BioFlux软件统计分析，结果显示CD45＋CD31＋BMSC与未分选的C57-BMSC粘附的DC数量上无差异，CD45-CD31-BMSC粘附的DC细胞明显减少。结论利用BioFlux数控剪切流条件下活细胞功能分析自动化系统可以直观观察细胞之间的粘附作用。Objective To establish a new method to observe the interaction between the marrow mesenchymal stem cells and DC cells. Methods BioFlux system is for live-cell assays under shear-flow .we first cultured C57 bone marrow mesenchymal stem cells （C57-BMSC）, CD45＋ CD31＋ BMSC and CD45-CD31-BMSC three kinds of cells in the microfluidic channels. DC cells were stained by Calcein-AM and added into the chamber under a shear force （0.5dynes/cm2）. Pictures were capured by fluorescence inverted. Data were analysised by BioFlux software. Results The number of DCs adhered with CD45＋ CD31＋ BMSC was no difference with unsorted C57-BMSC . The number of DCs adhered with CD45-CD31-BMSC significantly decreased than unsorted C57-BMSC. Conclusion Adhesion between cells can be visually observed by BioFlux system for live-cell assays under shear-flow.

关 键 词：流体剪切力 粘附作用 干细胞 树突细胞 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]