乙型肝炎病毒抗原抗体标志物与HBV-DNA联合检测的相关性分析  

Relative Analysis of Co-detection with Hepatitis B Virus Markers and HBV DNA

作　　者：秦昱[1] 夏先根 

机构地区：[1]四川省双流县第一人民医院,四川双流610210

出　　处：《河北医学》2014年第3期478-481,共4页Hebei Medicine

摘　　要：目的:分析乙肝五项及前S1抗原联合检测与HBV-DNA结果的一致性,探讨三者的相关性。方法:对508名门诊及住院病人的血清同时进行ELISA法检测乙肝五项、前S1抗原及荧光定量PCR法检测HBV-DNA,对检测结果进行对比分析。结果:乙肝五项不同模式间前S1抗原与乙肝DNA检测阳性率存在一定的差异,大三阳的前S1抗原和DNA检测阳性率分别为86.2%、100%,小三阳的检测阳性率分别为84.0%、92.0%,但具有一致性。结论:HBV前Sl抗原和HBV-DNA在各组中阳性检出率有较高的一致性,前Sl抗原在一定程度上可替代HBV-DNA。前Sl抗原与乙肝血清标志物联合检测能为乙肝患者病毒复制、肝功能损伤提供有价值的实验室依据,同时有助于慢性乙肝患者疗效考核和预后判断。Objective：To study the concordance of detection among HBV markers ,Pre S1 antigen and HBV-DNA, probe into the relativity between them .Method： Used Elisa to examine HBsAg , HBsAb, HBeAg HBeAb, HBcAb and preS1Ag of 508 patient＆#39;s sera, and PCR to examine HBV-DNA, then com-pared and analyzed the results .Result：Difference of positive rate existed between Pre S 1 antigen and HBV DNA among various mode of HBV markers .In the HBeAg positive row , preS1Ag positive ratio was 86.2%and that of HBV-DNA was 100%.Both preS1Ag and HBV-DNA reflected the activity and replication of HBV.In the HBeAg negative row , preS1Ag and HBV-DNA positive ratios were 84.0%and 92.0%.The positive ratio of HBV-DNA was consistent with preS1Ag in detection（P＆gt;0.05）.Conclusion：PreS1Ag can reflect the existence of HBV replication sensitively and can substitute HBV DNA .The diagnosis will be exact-ly if use the methods of preS 1Ag and hepatitis B serum markers in combination .

关 键 词：乙肝五项 前S1抗原 

分 类 号：R512.62[医药卫生—内科学]