响应面法优化瓜蒌薤白汤总黄酮的提取工艺  

Optimization of extraction technology for total flavonoids from Gualou Xiebai decoction by response surface methodolgy

作　　者：金东日[1] 洪波[1] 李宗亮[1] 张好好[1] 

机构地区：[1]吉林农业大学,资源与环境学院,吉林长春130118

出　　处：《食品工业科技》2014年第7期221-226,231,共7页Science and Technology of Food Industry

基　　金：吉林省科技发展计划项目(20110952,20130102078JC)

摘　　要：目的:研究提取复方瓜蒌薤白汤总黄酮的最佳工艺条件。方法:采用超声波和微波辅助提取瓜蒌薤白汤中总黄酮,并采用单因素和响应面法优化提取工艺。在乙醇浓度、料液比、时间、功率等单因素实验基础上,依据中心组合设计原理采用三因素三水平的响应面分析法进行最佳提取工艺的优化。结果:在分析各个因素的显著性和交互作用后,超声波最佳提取工艺条件为乙醇浓度50%,提取时间35min,料液比1∶50,在此条件下瓜蒌薤白汤黄酮的得率为5.168%;微波最佳提取工艺条件为料液比1∶50,提取时间1.5min,功率80W,黄酮得率为5.145%。结论:在此条件下,超声波辅助提取法提取黄酮得率较高。Objective： Research the optimum conditions of extracting flavonoids from Gualou Xiebai Decoction. Method：Ultrasonic and microwave was used to extract the total flavonoids, and the single factor and response surface methodology was selected to optimize extract process.On the basis of single factor experiments for the ethanol concentration, such as solid-liquid ratio, time, power, etc, the optimum conditions for total flavonoids extract were obtained through Box- Behnken center united experiment designed by response surface methodology.Result=The optimum conditions were as follows, the alcohol density was 50%, the extract/on time was 35min,and the solid-liquid ratio was 1：50. Under the conditions, the extraction rate of total flavonoids could be reached to 5.168%.Microwave extraction technology condition were as follows,the solid-liquid was 1：50,while the extract time was 1.Smin and 80W,flavonoids extraction yield could be reached by 5.145%.Conclusion.Within those conditions,ultrasonic assisted extraction to extract flavonoid yield is higher.

关 键 词：瓜蒌薤白汤 总黄酮 响应面分析法 超声波提取 微波提取 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]