盐酸右美托咪定对缺血-再灌注损伤大鼠心肌Toll样受体4/核因子-κB信号通路的作用  

Influence of dexmedetomidine on Toll-like receptor 4/Nuclear factor-κB signal pathway in rats with myocardial ischemia reperfusion injury by inhibiting

作　　者：蒋鹏[1] 黄祥君[2] 赵明[1] 陈盼[1] 马鹏 宗旭芳[1] 

机构地区：[1]江苏大学附属医院麻醉科,212001 [2]新疆生产建设兵团四师医院麻醉科

出　　处：《临床麻醉学杂志》2014年第3期294-296,共3页Journal of Clinical Anesthesiology

基　　金：新疆建设兵团卫生科技计划项目江苏大学临床医学科技发展基金(JLY20120162)

摘　　要：目的 研究盐酸右美托咪定对心肌缺血-再灌注损伤大鼠心肌组织中Toll样受体（toll-like receptor,TLR）-4 mRNA、核因子（nuclear factor,NF）-κB mRNA的表达,探讨盐酸右美托咪定对心肌缺血-再灌注损伤大鼠心肌TLR-4/NF-κB信号通路的影响.方法 健康3个月龄SD雄性大鼠21只,体重250～300 g,随机均分为三组：假手术组（sham组）、缺血-再灌注组（IR组）、缺血-再灌注＋盐酸右美托咪定组（DEX组）.采用结扎左冠状动脉前降支的方法制备大鼠心肌缺血-再灌注损伤模型,sham组左冠状动脉穿线不结扎.Sham组和IR组在结扎前30 min经腹腔注射生理盐水100 μg/kg,DEX组在结扎前30 min经腹腔注射盐酸右美托咪定注射液100 μg/kg（4 μg/ml）.实时定量逆转录聚合酶链反应（RT-PCR）方法检测TLR-4mRNA和NF-κB mRNA的表达.结果 sham、IR和DEX组的TRL-4mRNA分别为（2.21±0.11）、（7.83±0.35）和（3.91±0.21）,sham、IR和DEX组的NF-κB mRNA分别为（0.013±0.166）、（0.051±0.016）和（0.015±0.004）,IR、DEX组的TRL-4mRNA和NF-κB mRNA的表达明显高于sham组,且DEX组TRL-4mRNA和NF-κB mRNA的表达明显低于IR组（P＜0.05）.结论 盐酸右美托咪定可降低缺血-再灌注损伤大鼠心肌组织TLR-4mRNA和NF-κBmRNA的表达,盐酸右美托咪定可以调控TLR/NF-κB信号通路的转导,抑制炎症反应,减轻心肌缺血-再灌注损伤,保护心肌.Objective To investigate the influence of dexmedetomidine on expression of TLR-4 and NF-κB mRNA in rats with myocardial ischemia/reperfusion injury （IR）.Methods Twenty-one healthy Sprague-Dawley male rats,weighing 250-300 g,were randomly divided into 3 groups：group sham,group IR,and IR ＋Dexmedetomidine group （group DEX）.Left anterior descending coronary artery was ligatured to establish myocardial IR model.100 μg/kg dexmedotomidine was administered intraperitoneally 30 ming before ligature in grouop DEX,while the same volume of saline was administered in groups sham and IR.The expression of myocardial TLR-4mRNA and NF-κB mRNA were determined by real-time fluorescence quantitative（RT-PCR）.Results The TLR-4 rmRNA and NF-κB mRNA expression was significantly higher in groups IR and DEX compared with group sham （P＜0.05）.Compared with group IR,TLR-4 mRNA and NF-κB mRNA decreased in group DEX （P ＜0.05）.Conclusion Dexmedetomidine can reduce systemic inflammatory response,so that to protect myocardial ischemia / reperfusion injury through regulating TLR4/NF-κB signal pathway.

关 键 词：心肌缺血-再灌注损伤 TOLL样受体-4 核因子-ΚB 盐酸右美托咪定 

分 类 号：R614[医药卫生—麻醉学]