水分胁迫下马铃薯SnRK2基因的表达模式与生理响应  

SnRK2 Gene Expression in Responses to Physiological Characteristics under Water Stress in Solanum tuberosum

作　　者：毛娟[1,2] 白江平[1,2] 张俊莲[1,2] 范阿棋[1,2] 马宗桓[2] 吴金红[2] 王蒂[1,2] 

机构地区：[1]甘肃农业大学甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室,甘肃兰州730070 [2]甘肃农业大学农学院,甘肃兰州730070

出　　处：《中国沙漠》2014年第2期481-487,共7页Journal of Desert Research

基　　金：甘肃省干旱生境作物学重点实验室基金项目(GSCS-2012-04,GSCS-2010-16)资助

摘　　要：以"大西洋"和"陇薯3号"两个品种盆栽苗为试验材料,研究了水分胁迫下马铃薯(Solanum tuberosum)StSnRK2基因家族的表达特征及其与植株生理指标变化的相关性。结果表明:在发生严重水分胁迫时,"陇薯3号"和"大西洋"的抗氧化酶活性、MDA含量、相对电导率和脯氨酸含量均较对照显著提高,但增长幅度在品种间存在差异;StSnRK2基因家族的8个成员的相对表达量在"大西洋"和"陇薯3号"之间也存在明显差异,StSnRK2.1、StSnRK2.2和StSnRK2.3在两个品种中均表现为水分胁迫后相对表达量显著高于对照的趋势;StSnRK2.7的相对表达量与对照无显著差异,而StSnRK2.4在"陇薯3号"的相对表达量是对照的9.5倍,是8个基因成员在受水分胁迫后相对表达量最高的一个基因;StSnRK2.1、StSnRK2.2、StSnRK2.3、StSnRK2.4和StSnRK2.6的相对表达量均与部分生理指标呈极显著正相关,StSnRK2.5和StSnRK2.8两个基因的相对表达量与部分生理指标呈极显著负相关,StSnRK2.7基因的相对表达量与生理指标无显著相关性。The physiological response and StSnRK2 expression profiles of two solanum tuberosum （Longshu No.3 and Atlantic） were studied after water stress treatments. The physiological character of the acticivies of anti-oxcide enzymes, malondialdehyde content, relative conductivity and proline content changed, but the two cultivars had different characters. The relative expression levels of 8 members in Atlantic and Longshu No.3 had obvious difference after severe water stress. The relative expression of StSnRK2.1, StSnRK2.2 and StSnRK2.3 were significantly higher than the control, however, obvious difference was found in an increase extent of different gene expression. StSnRK2.7 had no difference with the control. The expression of StSnRK2.4 was 9.5 fold in Longshu No.3, which was the maximum among the 8 members after water stress. The expression of StSnRK2.1, StSnRK2.2, StSnRK2.3, StSnRK2.4 and StSnRK2.6, had a high significantly positive correlation with some of the physiological characteristics. The significantly negative correlation was observed between the expression of StSnRK2.5 and StSnRK2.8 and some of the physiological characteristics. Expression of StSnRK2.7 had no relationship of physiological characteristics.

关 键 词：干旱胁迫 马铃薯(Solanum tuberosum) SnRK2 基因表达 生理响应 

分 类 号：Q945.79[生物学—植物学]