添加SNP改变储存红细胞血流变学和一氧化氮含量研究  

Effects of SNP on hemorheology and nitric oxide levels in stored erythrocyte

作　　者：解绪红[1] 姚慧青[1] 杨秀华[1] 魏亚明[1] 

机构地区：[1]广州医科大学附属广州市第一人民医院输血科,广东广州510180

出　　处：《中国输血杂志》2014年第2期136-139,共4页Chinese Journal of Blood Transfusion

基　　金：广东省自然基金(S2012010009016);广东省医学科研基金(A2013500)

摘　　要：目的探索储存红细胞添加NO供体SNP后血液流变学各项指标以及红细胞内胞浆NO、总NO、Hb结合NO含量变化,并与新鲜红细胞内3种NO含量比较,探索其可能的临床意义。方法血样采自10名健康献血者,制成悬浮红细胞后分为新鲜红细胞组和储存红细胞组。新鲜红细胞组滤除白细胞后检测红细胞内3种NO含量。储存红细胞组储存d20时滤除白细胞,分为2部分,1部分与终浓度分别为1、10μmol/L的SNP孵育,检测流变学各项指标;另1部分与终浓度分别为0.2、1、5、25μmol/L的SNP孵育,检测红细胞内3种NO含量。结果当储存红细胞添加1μmol/L SNP时,各项流变学指标与未加SNP组相比均无统计学意义(P>0.05);当添加10μmol/L SNP时,红细胞低切粘度、中切粘度、还原低切粘度、还原中切粘度、聚集指数、电泳指数分别为4.16±0.74、2.94±0.41、9.41±1.19、5.92±0.48、1.64±0.10、4.30±0.28,均显著低于未加SNP组(P<0.05)。新鲜红细胞内3种NO含量分别是11.09±2.95、16.63±4.46、5.54±3.53,储存红细胞(未加SNP组)3种NO含量分别是4.93±1.01、7.20±0.93、2.27±1.18,后者3种NO含量均明显低于前者(P<0.05)。当储存红细胞与0.2μmol/L SNP孵育时,3种NO含量与未加SNP组比较均无统计学差异(P>0.05);当增加至1μmol/L时,3种NO含量分别是5.93±1.41、9.57±1.56、3.74±1.36,均显著高于未加SNP组(P<0.05);当增加至5μmol/L时,总NO、Hb结合NO含量分别为8.45±2.12、3.29±1.54,继续增加至25μmol/L时,总NO、Hb结合NO含量分别为8.86±1.16、3.65±0.91,均明显高于未加SNP组(P<0.05)。结论添加适当浓度NO供体SNP可以明显改善储存红细胞变形性,显著增加红细胞内NO含量。Objective To detect hemorheology and cytoplasm NO,total NO and NO-hemoglobin levels in erythroeytes after adding different concentrations of SNP to stored suspension red blood cells（RBCs）. Methods Blood samples were collected from l0 healthy donors, then divided into fresh and 20 days stored suspension RBCs groups. Hemorheology was assayed by cone-plate rotating structure principle. Cytoplasm NO and total NO were detected hy colorimetric analysis. The level of NO-hemoglobin was equal to the level of total NO minus cytoplasm NO. The NO levels of fresh red blood cells were detected with above methods. At 20 days,stored red blood cells were divided into two parts. Part one was incubated with SNP at 1 μmoL/L and 10 μmol/L concentrations for 1 hour and hemorheol0gy was assayed with the above method. Part two was incubated with SNP at 0.2,1,5 and 25 μmol/L concentrations for 1 hour and the NO levels were detected with above methods. Results When stored erythrocytes were incubated with 10 μ mol/L SNP,the viscosity in low,middle shear,in- dex of suspension erythrocytes aggregation and electophoresis index were 4.16 ±0.74,2.94±0.41,9.41 ± 1.19,5.92 ± 0.48,1.64± 0.10 ,and 4.30± 0.28 respectively. Values obtained were significantly lower than the group without SNP ad- dition （ P 〈 0. 05 ）, while 1 μmol/L SNP showed no effect on hemorheology （P 〉 0. 05 ）. For fresh red blood ceils, the levels of cytoplasm NO, total NO and NO-hemoglobin were 11.09±2.95,16.63± 4.46,5.54 ±3.53, and stored ceils were 4.93 ± 1.01,7.20± 0.93,2.27± 1.18 ,respectively. The stored groups had NO levels significantly lower than fresh ones. At 0. 2 μmol/L SNP incubation, the three NO levels did not change compared with the 20 days stored group （P 〉 0. 05 ） and when SNP concentration went UP to 1 p.mol/L,the three NO levels were 5.93 ± 1.41,9.57±1.56,3.74,±1.36. Significant differences were found compared to the control group （ P 〈 0. 05 ）, when SNP was added to 5 μmol/L, the total NO and NO- hemoglobin l

关 键 词：一氧化氮 储存红细胞 血液流变学 Hb结合NO 

分 类 号：R457.1[医药卫生—治疗学]