多孔阳极氧化铝表面蛋白吸附及其释放行为  

The Absorption and Release Behaviors of BSA and BF on Porous Anodic Alumina Surface

作　　者：吴培[1] 李晓宏[1] 倪似愚[1] 

机构地区：[1]东华大学化学化工与生物工程学院,上海201620

出　　处：《东华大学学报（自然科学版）》2014年第1期76-82,共7页Journal of Donghua University(Natural Science)

基　　金：国家自然科学基金资助项目(50902020)

摘　　要：采用两步阳极氧化法在草酸电解液中制备出孔径为75nm的多孔阳极氧化铝(PAA),考察了牛血清白蛋白(BSA)和牛血纤维蛋白(BF)在PAA表面的吸附和释放行为.采用BCA(bicinchoninic acid)法定量检测蛋白的吸附和释放量,采用Langmuir等温拟合曲线模拟蛋白的吸附行为,通过场发射扫描电镜(FESEM)、X射线能谱仪(EDS)和傅里叶红外光谱(FTIR)对蛋白吸附前后样品的微观形貌和成分进行了表征.试验结果表明:与平滑铝相比,PAA显著提高了两种蛋白在其表面的吸附能力,且BF的吸附量明显高于BSA,两者的最大吸附量分别达到60.3和38.8μg/cm2;PAA对两种蛋白的吸附符合Langmuir等温吸附模型;PAA吸附蛋白后的释放行为分为突释和缓释两个阶段,其吸附BSA和BF的最大释放率分别达到55%和65%.Porous anodic aluminum（PAA） with nanopore diameter of 75 nm was fabricated by two steps anodic oxidation technique in oxalic acid. The adsorption and release behavior of bovine serum albumin （BSA） and bovine fibrinogen （BF） on the surfaces of PAA samples were researched. The protein adsorption and release quantity were measured by using the bicinchoninic acid （BCA） protein assay method. Langmuir isothermal fitting curve was used to simulate the adsorption of protein. The micromorphology and components of the PAA samples before and after protein absorbing were characterized by field emission scanning electron microscopy （FESEM）, X-ray energy dispersive spectroscopy（EDS） and Fourier transform infrared spectroscopy（FTIR）, respectively. The research results indicated that the adsorption of BSA and BF onto the surface of PAA was significantly enhanced as compared to the flat aluminum. Meanwhile, the adsorption quantity of BF was obviously higher than that of BSA and the saturation adsorption quantity of BSA and BF was 38. 8 and 60. 3 t^g/cm2, respectively. The adsorption behavior of BSA and BF to the surface of PAA was in accordance with Langmuir isothermal adsorption model. The process of protein release of the PAA surface included two stages, burst release and slow release, the greatest release rate of BSA and BF was 55% and 65%, respectively.

关 键 词：多孔阳极氧化铝 牛血清蛋白 牛血纤维蛋白 吸附和释放 

分 类 号：TB34[一般工业技术—材料科学与工程] TQ131.11[化学工程—无机化工]