贵州8种树材对天麻蜜环菌生长的影响  

Effect of Eight Different Tree Species on Armillaria mellea Growth of Gastrodia elatain Guizhou

作　　者：黄明进[1,2] 罗春丽[1,2] 刘红昌[1,2] 赵致 

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]贵州省中药材繁育与种植工程实验室,贵州贵阳550025

出　　处：《贵州农业科学》2014年第2期115-118,共4页Guizhou Agricultural Sciences

基　　金：贵州省科技厅中药现代化项目[黔科合ZY(2011)3011];贵州省农业动植物育种专项[黔农育专字(2011)030];国家科技支撑计划子课题(2011BAI13B04-03)

摘　　要：为寻找更多适合天麻生产上培养蜜环菌的树材,选取桉树、天竺桂和女贞等8种贵州常见树材的枝或干作菌材接种蜜环菌,观察培养蜜环菌在生长过程中的接菌时间、色泽变化、顶端分生能力、菌索直径及其密集程度等,并对各菌材所培育出的蜜环菌菌索进行HPLC指纹图谱比较分析。结果表明:8种树材10d左右均可长出蜜环菌菌索,其色泽基本一致,幼嫩时呈黄白色或浅黄色,成熟后呈棕色,老化之前尖端都有白色的生长点,老化后呈黄褐色、深褐色或黑褐色,菌索顶端分生组织在老化前分裂能力较强;8种树材中,马尾松作为蜜环菌菌种接种菌材的菌索直径最大,天竺桂菌材最小;菌索数量,滇鼠刺菌材最多,天竺桂菌材最少;马尾松易腐烂、虫蛀。不同菌材培养的蜜环菌菌索的HPLC指纹图谱有一定的差异。8种树材中,滇鼠刺、女贞和天竺桂较适合作为培育蜜环菌的菌材。The inoculated time,color change,apical meristem capacity,rhizomorph diameter and dense degree of Armillaria mellea inoculated on branches and trucks of eight common tree species were observed and the finger-print of Armillaria mellea rhzomorph from different tree species was analyzed by HPLC to seek more suitable tree species for A.mellea growth in Gastrodia elata production.The results showed that A.mellea rhizomorph with the same color basically could emerge on eight tree species after 10d all and the color of young and matured A.mellea rhizomorph was yellowish white or pale yellow and brown.There were white growing points on the tip of A.mellea rhizomorph before ageing of A.mellea rhzomorph.The color of ageing A.mellea rhizomorph was yellowish-brown,dark brown or black brown. The apical meristem of A.mellea rhzomorph was of the strong fission ability before its ageing.The rhzomorph diameter of A.mellea inoculated on Pinus massoniana and C.pedunculatum was maximum and minimum.The rhzomorph number of I .yunnanensis and C.pedunculatum was maximum and minimum. There was a certain difference in HPLC finger-print of A.mellea rhzomorph among different inoculated on different tree species.I .yunnanensis,Ligustrum lucidum and C.pedunculatum are suitable materials for culture of A.mellea.

关 键 词：天麻菌材 蜜环菌 指纹图谱 贵州 

分 类 号：S567.239[农业科学—中草药栽培]