平胃散中苍术素在大鼠体内药代动力学研究  

Determination of Atractylodin of Pingwei Powder in rat plasma by HPLC-UV method and their application to a pharmacokinetic and a tissue distribution study

作　　者：李古月[1,2] 刘佳鑫[1,2] 才谦[3] 

机构地区：[1]中国医科大学附属第一医院,辽宁沈阳110000 [2]中国医科大学药学院,辽宁沈阳110000 [3]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中国医药导报》2014年第8期19-22,共4页China Medical Herald

基　　金：国家自然科学基金资助项目(编号81202919);国家中医药管理局中医药行业科研专项项目(编号20110700 712)

摘　　要：目的研究平胃散中苍术素在大鼠血浆及组织中分布特征。方法建立大鼠血浆中苍术素的HPLC测定方法,考查大鼠口服平胃散混悬液0.17,0.33,0.5,1.0,2.0,3.0,4.0,6.0,8.0,12,24 h后血药浓度变化和2,3,8 h后大鼠体内组织分布情况,采用DAS 2.0数据处理软件计算药代动力学参数。结果大鼠口服平胃散混悬液后,苍术素在大鼠体内的达峰时间Tmax为2.5 h,最大浓度Cmax为1.765 mg/L,半衰期t1/2z为5.723 h,AUC 11.789 mg/L·h,且在6 h左右时第2次达峰,出现双峰现象。大鼠口服平胃散混悬液后2,3,8 h的药物组织分布由高到低的顺序大致是胃>小肠、脾>肝、肺、心>肾、大肠。结论该方法灵敏、快速、准确,可用于大鼠血浆中苍术素浓度的测定。Objective To study the blood concentration and tissue distribution of Atractylodin after oral administration of Pingwei Powder to rats.Methods The determination methods of Atractylodin in Pingwei Powder in rat plasma was established by HPLC,the blood concentration of Atractylodin after oral administration of Pingwei Powder at 0.17,0.33,0.5,1.0,2.0,3.0,4.0,6.0,8.0,12,24 h and tissue distribution in rats at 2,3,8 h were examined,the data was dealt with DAS 2.0 data processing software to calculate the pharmacokinetics parameters.Results After oral administration of Pingwei Powder,the Tmax was 2.5 h,Cmax was 1.765 mg/L,t1/2z was 5.723 h,AUC was 11.789 mg/L·h.The second peak was appeared at 6 h.After oral administration of Pingwei Powder,Atractylodin was quickly distributed to main organs with the concentration difference,stomach 〉 small intestine,spleen 〉 liver,lung,heart 〉 kidney,large intestine.Conclusion This paper has developed a simple,specific,and rapid method of RP-HPLC with UV detection to determine Atractylodin of Pingwei Powder in rat plasma.

关 键 词：平胃散 苍术素 药代动力学 组织分布 高效液相色谱 

分 类 号：R285[医药卫生—中药学]