酶联免疫斑点试验诊断结核性胸膜炎的临床应用价值  

Clinical application value of ELISPOT in the diagnosis of tuberculous pleuritis

作　　者：董璇[1] 李天兴[2] 韩晓群[3] 黄汉平[4] 

机构地区：[1]武汉市医疗救治中心结核性脑膜炎科,湖北武汉430012 [2]武汉市医疗救治中心胸外科,湖北武汉430012 [3]武汉市医疗救治中心感染科,湖北武汉430012 [4]武汉市医疗救治中心结核呼吸科,湖北武汉430012

出　　处：《中国医药导报》2014年第8期91-93,共3页China Medical Herald

基　　金：湖北省武汉市卫生局科研课题(编号WX12C15)

摘　　要：目的 探讨酶联免疫斑点试验（ELISPOT）诊断结核性胸膜炎的临床应用价值.方法 选择2011年8月～2012年8月武汉市医疗救治中心住院21例结核性胸膜炎患者（结核组）和18例肺癌胸膜转移患者（恶性肿瘤组）,利用ELISPOT法检测两组患者胸水对结核特异性抗原刺激产生反应,分泌γ干扰素的效应T淋巴细胞的阳性率.结果 ELISPOT结果显示结核组胸水中T淋巴细胞检测阳性率为85.7％（18/21）,明显高于恶性肿瘤组[16.7％（3/18）],差异有统计学意义（P＜0.05）.ELISPOT的敏感性与特异性分别为85.7％、83.3％.结论 ELISPOT灵敏性和特异性较高,对于结核性胸膜炎具有一定的临床诊断价值.Objective To investigate the clinical application value of ELISPOT in the diagnosis of tuberculous pleuritis.Methods 21 patients with tuberculous pleuritis in Wuhan Medical Treatment Center from August 2011 to August 2012 were selected as tuberculosis group,18 patients with lung cancer and pleural metastasis were selected as malignant tumor group.ELISPOT method was used to detect the pleural effusion,peripheral blood in tuberculosis group and pleural effusion in malignant tumor group; the tuberculosis specific antigen stimulation reactions was observed; the positive rate of T lymphocyte with secreting gamma interferon were observed.Results T lymphocyte testing positive rate in tuberculosis group 85.7 % （18/21）,significant higher than that of tuberculosis group [16.7 % （3/18）] according to the ELISPOT results,the difference was statistically significant （P 〈 0.05）.The sensitivity and specificity of ELISPOT were 85.7% and 83.3% repectively.Conclusion ELSPOT is a sensitive and specific detection method of tuberculosis pleuritis,it has clinical diagnostic value.

关 键 词：结核性胸膜炎 酶联免疫斑点试验 诊断 

分 类 号：R446.61[医药卫生—诊断学]