毛竹等28个刚竹属竹种的AFLP分析  

AFLP analysis of 28 Phyllostachys bamboo species including Moso bamboo

作　　者：李雪平[1] 牟少华[1] 彭镇华[2] 高健[1] 吴惠俐[1] 

机构地区：[1]国际竹藤中心国家林业局竹藤科学与技术重点开放实验室,北京100102 [2]中国林业科学研究院林业研究所,北京100091

出　　处：《安徽农业大学学报》2014年第2期179-182,共4页Journal of Anhui Agricultural University

基　　金：国际竹藤网络中心基本科研业务费重点项目(1682011004)资助

摘　　要：利用AFLP分子标记技术对毛竹等28个刚竹属的竹种进行了遗传多样性分析,共筛选出14对多态性好、清晰度较高的引物组合,获得785个位点,其中多态性位点654个,占总位点的83.31%。利用POPGENE1.32软件对AFLP数据进行分析,结果表明,观测等位基因数为1.8303,有效等位基因数为1.4211,Nei’s基因多样性指数为0.2551,Shannon信息指数为0.3913,并根据遗传距离进行UPGMA聚类分析。Amplified fragment length polymorphism （AFLP） was used to analyze the genetic diversity of 28 Phyllostachys bamboo species including Moso bamboo. Fourteen primer pairs with higher polymorphic were se- lected. 785 loci were examined for molecular variation and 654 loci were polymorphic, accounted for 83.31% of total loci. The AFLP data were analyzed using POPGENE 1.32 software, and the results showed as follows. The observed number of alleles was 1.8303; the effective number of alleles was 1.4211; the Nei＇s gene diversity index was 0.2551; Shannon information index was 0.3913, and the 28 bamboo species were divided according to the UPGMA cluster analysis.

关 键 词：刚竹属 AFLP 遗传多样性 

分 类 号：S795.7[农业科学—林木遗传育种]