不同类型雄性不育苎麻细胞学机理研究  

Cytological mechanism of sterility in different types of male sterile ramie

作　　者：薛丽君[1] 邢虎成[1,2] 马艳玲[1] 

机构地区：[1]湖南农业大学苎麻研究所,湖南长沙410128 [2]湖南省种质资源创新与资源利用重点实验室,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2014年第1期13-18,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金青年项目(31101098);湖南省自然科学基金青年项目(11JJ4025)

摘　　要：为探明苎麻雄性不育材料和可育材料在花芽分化和花药发育上的差异，选取不可育材料雄化萎缩型同青5号、花被不开裂型弯子苎麻、花粉不育型黄小叶和可育材料中苎1号（对照），采用常规石蜡切片技术对其进行雄花花芽发育形态比较。结果表明：3种雄性不育苎麻花芽发育较可育苎麻中苎1号快，在花芽发育到0．3～0．5cm时，已完成了雌、雄蕊的分化。参照可育苎麻材料的花芽分化和花药发育，推测3种雄性不育材料不育的原吲分别为园青5号花芽在分化出花药和花丝后，花药不会继续分化形成花粉囊，而是变形萎缩甚至消失形成空腔；弯子苎麻在花粉分化阶段花被木质化严重；黄小叶花粉囊不破裂，花粉粒颜色逐渐变深．花丝发育异常。To investigate the differences in flower bud differentiation and anther development between male sterile and fertile materials of ramie, male steril ramie including male flower atrophic type （Yuanqing No.5）, male flower indehiscent type （Wanzi ramie）, pollen sterility type （Huangxiaoyie） and fertile ramie Zhong Zhu No. 1 （control） were selected to compare the development of male flower buds by paraffin section technology. The results showed that flower buds of male steril ramie develop faster than Zhongzhu No. 1. When the flower buds are 0.3-0.5 cm long, the differentiation of pistil and stamen are completed. It is speculated according to the differentiation of flower buds and anther development of Zhong Zhu No.1 that the cause of male steril for Yuanqing No.5 was after differentiation of anther and filament, anther wouldn＇t differentiate to form pollen sac, but undergo atrophy, or even disappear and form cavity; for Wanzi Ramie, the cause was serious lignified perianth in the stage of pollen differentiation, for huangxiaoyie the unruptured pollen sac, abnormal intumescence of filament and the darkened colour of pollen grain.

关 键 词：苎麻 雄性不育 细胞学 石蜡切片观察 

分 类 号：S563.101[农业科学—作物学]