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作 者:沈素贞[1] 聂东伶[2] 吴思政[2] 梁文斌[1] 柏文富[2]
机构地区:[1]中南林业科技大学生命科学院,湖南长沙410004 [2]湖南省森林植物园,湖南长沙410116
出 处:《湖南农业大学学报(自然科学版)》2014年第1期28-31,共4页Journal of Hunan Agricultural University(Natural Sciences)
摘 要:采用盆栽试验,将广东万年青在25(对照)、30、35、40、45℃下分别处理8 d后,在常温(25℃)下恢复3 d,于2,4,6,8,11 d取样检测叶片质膜相对透性、丙二醛含量、POD活性及可溶性糖含量的变化。结果表明:叶片质膜相对透性在低于35℃处理后的变化幅度不大,在40、45℃高温胁迫下随时间的延长增幅变大,最大增幅可达72.62%;丙二醛含量在高温胁迫初期明显上升,且随胁迫温度的升高增幅变大,常温恢复3 d后高于35℃胁迫处理的丙二醛含量未下降;过氧化物酶活性在高于35℃胁迫处理下先升高,至胁迫第6天开始下降,且胁迫温度越高的处理的酶活性变化越大,常温恢复3 d后高于40℃胁迫处理的酶活性继续下降;可溶性糖含量在高于35℃高温胁迫处理的初期迅速积累,在处理中期达到高峰,然后下降,常温恢复3 d后含量继续下降。Based on pot experiment, Aglaonema modestum Schott was treated under the temperature of 25(control), 30, 35, 40, 45 ℃ respectively for 8 d and then in normal temperature (25 ℃) for 3 d. The changes of relative permeability of plasma membrane, malondialdehyde (MDA) content, peroxidase (POD) activities and soluble sugar content of Aglaonema modestum Schott leaves collected on day 2, 4, 6, 8 and 11 were tested. The results indicated that the relative permeability of plasma membrane kept relatively steady in 35 ℃ and increased with the time under 40 ℃ and 45 ℃ high temperature stress, the most increase could be up to 72.62%; MDA content increased obviously with the temperature at the earlier stage of stress and did not decline after the temperature restored to room level for 3 d at those treatments in which the temperature was above 35 ℃; POD activities increased at the earlier stage of stress in the treatments of the temperature was higher than 35 ℃, and then declined after 6 days later, and the higher the temperature, the greater the change of POD activities in all treatments, and POD activities kept decreasing under the temperature restored to room level for 3 d among the treatments in which the temperature was above 40 ℃ stress; soluble sugar content increased obviously at the earlier stage of stress and reached a peak at the middle stage, and then declined at the temperature restored to room level for 3 d among the treatments which the temperature was above 35 ℃ stress.
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