瘰疬膏的质量控制方法  

Quantitative and qualitative study on Luoligao

作　　者：陈鹏英[1] 

机构地区：[1]武汉市中医医院药学部,湖北武汉430014

出　　处：《中国医院药学杂志》2014年第6期480-483,共4页Chinese Journal of Hospital Pharmacy

基　　金：武汉市卫生局科技攻关项目(编号:武卫[2005]294号)

摘　　要：目的：建立瘰疬膏的定性定量方法。方法：采用薄层色谱（TLC）法对瘰疬膏中的主要中药玄参、夏枯草进行定性鉴剐；采用高效液相色谱（HPLC）法测定瘰疬膏中迷迭香酸的含量。色谱柱：VP-ODSC，。柱（250mmx4．6mm，5Mm）；流动相：乙腈-0．1％磷酸梯度洗脱（0→15min，20：80；15—34min，20→18：80→82；34→35min，18—20）：82—80，恢复起始浓度比）；检测波长=330nm；流速：1．0ml·min^-1；柱温：30℃。结果：薄层色谱斑点清晰，阴性样品无干扰；HPLC出峰时间在34min左右。迷迭香酸含量测定在0．0632～0．316μg与峰面积呈良好的线性关系。回归方程为Y=33703 227．85X-2970．0，r=0．9999（n=5），平均回收率为103．7%，RSD=0．38％（n=6）。结论：本方法简便、快捷、准确，专属性强，重复性好，阴性无干扰，可作为瘰疬膏的质量控制方法。OBJECTIVE To establish a quantitative and qualitative method of identification for Luoligao. METHODS Radix Scrophulariae and Spica Prunelae were identified by TLC in Luoligao. The content of rosmarinie acid was determined by HPLC. The chromatographic column was Eclipse SHIMADZU VP-ODS C18 （250 mm × 4. 6 mm, 5μm）. The mobile phase was acetoni- trile-0. 1 % phosphoric acid with gradient. The column temperature was set at 30 ℃ The flow rate was 1.0 ml. min^-1 and the UV detection wavelength was 330 nm. RESULTS The quality identification by TLC was specific, well, separated and a clear spot in each thin Iayer plate,and the calibration curve for rosmarinie acid was in good linearity within 0. 063 2 - 0. 316μg. The regression equation was Y = 33 703 227. 85X- 2 970. 0, r = 0. 999 9 （n = 5）, The average recovery was 103.7 %, with RSD = 0. 38% （n = 6）. CONCLUSION The method is rapid,simple,accurate,and can be used for the quality control of Luoligao.

关 键 词：瘰疬膏 玄参 夏枯草 哈巴俄苷 迷迭香酸 薄层色谱法 高效液相色谱法 

分 类 号：R286[医药卫生—中药学]